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Literature summary for 3.4.21.39 extracted from

  • Takao, K.; Takai, S.; Shiota, N.; Song, K.; Nishimura, K.; Ishihara, T.; Miyazaki, M.
    Lack of effect of carbohydrate depletion on some properties of human mast cell chymase (1999), Biochim. Biophys. Acta, 1427, 74-81.
    View publication on PubMed
Search for more literature for 3.4.21.39

General Stability

General Stability Organism
no change in susceptibility to autolysis due to deglycosylation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0141
-
 big endothelin deglycosylated enzyme Homo sapiens
0.0145
-
 Big endothelin-1 glycosylated enzyme Homo sapiens
0.0223
-
 angiotensin I deglycosylated enzyme Homo sapiens
0.0255
-
 angiotensin I glycosylated enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification carbohydrate content is 10%. The N-glycan contributes to the stability of the enzyme but not to its functional properties Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
artery gastroepiploic Homo sapiens
-
mast cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
 Homo sapiens angiotensin II + His-Leu
-
 ?
big endothelin + H2O
-
 Homo sapiens ?
-
 ?
Big endothelin-1 + H2O
-
 Homo sapiens ?
-
 ?
collagen type IV + H2O
-
 Homo sapiens ?
-
 ?
Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
interleukin 1beta precursor + H2O
-
 Homo sapiens ?
-
 ?
neurotensin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 deglycosylated enzyme Homo sapiens
50 55 glycosylated enzyme Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 pH 7.4, glycosylated enzyme, stable for 24 Homo sapiens
40
-
 pH 8.0, 20 min, stable up to Homo sapiens
50
-
 pH 8.0, 20 min, about 65% loss of activity, untreated enzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.7
-
 Big endothelin-1 untreated enzyme Homo sapiens
25.6
-
 Big endothelin-1 deglycosylated enzyme Homo sapiens
140
-
 angiotensin I glycosylated enzyme Homo sapiens
143
-
 angiotensin I deglycosylated enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 3 h, stable Homo sapiens
7.4
-
 37°C, glycosylated enzyme, stable for 24 Homo sapiens