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Literature summary for 3.4.21.39 extracted from

  • Thorpe, M.; Fu, Z.; Albat, E.; Akula, S.; de Garavilla, L.; Kervinen, J.; Hellman, L.
    Extended cleavage specificities of mast cell proteases 1 and 2 from golden hamster Classical chymase and an elastolytic protease comparable to rat and mouse MCP-5 (2018), PLoS ONE, 13, e0207826 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.39

Activating Compound

Activating Compound Comment Organism Structure
enterokinase
-
 Mesocricetus auratus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baculovirus-infected insect cells Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin-Sepharose column chromatography Mesocricetus auratus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with succinyl-AAPL-4-nitroanilide, succinyl-AAPI-4-nitroanilide, succinyl-AAPA-4-nitroanilide, succinyl-AAPV-4-nitroanilide, succinyl-VLGR-4-nitroanilide, benzyloxycarbonyl-GPR-4-nitroanilide, acetyl-YVAD-4-nitroanilide, acetyl-VEID-4-nitroanilide, and acetyl-IEPD-4-nitroanilide Mesocricetus auratus ?
-
 ?
additional information the enzyme does not have a particular preference for any of the three aromatic amino acids, Phe, Tyr and Trp, in the P1 position of substrates and also efficiently cleaves after Leu (with a 3fold lower cleavage activity on Leu compared to substrates with P1 aromatic amino acids). The enzyme has a strong preference for negatively charged amino acids in the P2' position Mesocricetus auratus ?
-
 ?
succinyl-AAPF-4-nitroanilide + H2O
-
 Mesocricetus auratus succinyl-AAPF + 4-nitroaniline
-
 ?
succinyl-LLVY-4-nitroanilide + H2O
-
 Mesocricetus auratus succinyl-LLVY + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Mesocricetus auratus

Synonyms

Synonyms Comment Organism
HAM1
-
 Mesocricetus auratus
mast cell protease 1
-
 Mesocricetus auratus