Cloned (Comment) | Organism |
---|---|
enterokinase cleavage site and His6-tag, expressed in HEK 293-EBNA cell | Canis lupus familiaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE (active enzyme) | Canis lupus familiaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Canis lupus familiaris |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), activation by His6-tag removal by cleavage with enterokinase, heparin-Sepharose chromatography | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mast cell | - |
Canis lupus familiaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methoxy-succinyl-Arg-Ala-Tyr-4-nitroanilide + H2O | cleavage specificity as determined by substrate phage display P4: (Gly)Val, P3: Gly/Val, P2: Arg > Gly, P1: Phe > Tyr > Trp, P1': Leu > Val, P2': Ser > Glu/Asp > Leu, P3': Leu/Val | Canis lupus familiaris | methoxy-succinyl-Arg-Ala-Tyr + 4-nitroaniline | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Canis lupus familiaris | N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE (active enzyme) | Canis lupus familiaris |
Synonyms | Comment | Organism |
---|---|---|
chymase | - |
Canis lupus familiaris |