Literature summary for 3.4.21.39 extracted from

Andersson, M.K.; Karlson, U.; Hellman, L.
The extended cleavage specificity of the rodent beta-chymases rMCP-1 and mMCP-4 reveal major functional similarities to the human mast cell chymase (2008), Mol. Immunol., 45, 766-775.

Search for more literature for 3.4.21.39

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in 293-EBNA cells	Mus musculus
expressed in 293-EBNA cells	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	SDS-PAGE	Mus musculus
27000	-	SDS-PAGE	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose bead chromatography and heparin-Sepharose column chromatograohy	Mus musculus
Ni-NTA agarose bead chromatography and heparin-Sepharose column chromatograohy	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methoxy-succinyl-Arg-Ala-Tyr-4-nitroanilide + H2O	-	Mus musculus	methoxy-succinyl-Arg-Ala-Tyr + 4-nitroaniline	-	?
methoxy-succinyl-Arg-Ala-Tyr-4-nitroanilide + H2O	-	Rattus norvegicus	methoxy-succinyl-Arg-Ala-Tyr + 4-nitroaniline	-	?
additional information	prefers Phe in the P1 position (56%), followed by Tyr (28%) and Trp (16%), position P2 is most often occupied by Leu (22%), in positions P3 Val is the preferred amino acid, in position P4 is a preference for Val (20%) and Trp (19%) over other hydrophobic amino acids	Mus musculus	?	-	?
additional information	prefers Phe in the P1 position (59%), followed by Trp (29%) and Tyr (12%), position P2 is most often occupied by Leu (24%) or Arg (22%), in positions P3 and P4 Val is the preferred amino acid	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
beta-chymase	-	Mus musculus
beta-chymase	-	Rattus norvegicus
MCP-1	-	Rattus norvegicus
MCP-4	-	Mus musculus

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Release 2023.1 (January 2023)