Cloned (Comment) | Organism |
---|---|
mutant is expressed in Escherichia coli BL21(DE3) cells | Cavia porcellus |
Protein Variants | Comment | Organism |
---|---|---|
A216G | the partially humanized mutant acquires human-like P1 Phe- and angiotensin-cleaving capacity | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha1-Aantichymotrypsin | - |
Cavia porcellus | |
alpha1-Aantichymotrypsin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
Ala-Ala-Pro-Phe-4-nitroanilide | wild type enzyme | Homo sapiens | |
0.53 | - |
Ala-Ala-Pro-Phe-4-nitroanilide | wild type enzyme | Cavia porcellus | |
0.65 | - |
Ala-Ala-Pro-Phe-4-nitroanilide | mutant enzyme A216G | Cavia porcellus | |
0.97 | - |
Ala-Ala-Pro-Met-4-nitroanilide | wild type enzyme | Cavia porcellus | |
1.02 | - |
Ala-Ala-Pro-Leu-4-nitroanilide | wild type enzyme | Homo sapiens | |
1.5 | - |
Ala-Ala-Pro-Leu-4-nitroanilide | mutant enzyme A216G | Cavia porcellus | |
1.55 | - |
Ala-Ala-Pro-Leu-4-nitroanilide | wild type enzyme | Cavia porcellus | |
3 | - |
Ala-Ala-Pro-Met-4-nitroanilide | wild type enzyme | Homo sapiens | |
27 | - |
Ala-Ala-Pro-Met-4-nitroanilide | mutant enzyme A216G | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | A7WPL7 | precursor | - |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Cavia porcellus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Pro-Leu-4-nitroanilide + H2O | - |
Homo sapiens | Ala-Ala-Pro-Leu + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Leu-4-nitroanilide + H2O | - |
Cavia porcellus | Ala-Ala-Pro-Leu + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Met-4-nitroanilide + H2O | - |
Homo sapiens | Ala-Ala-Pro-Met + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Met-4-nitroanilide + H2O | - |
Cavia porcellus | Ala-Ala-Pro-Met + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Homo sapiens | Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Phe-4-nitroanilide + H2O | negligible activity | Cavia porcellus | Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
angiotensin I + H2O | - |
Homo sapiens | angiotensin II + ? | - |
? | |
additional information | lacks elastase-like preference for P1 Val or Ala, and does not cleave angiotensin I | Cavia porcellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-chymase | - |
Homo sapiens |
alpha-chymase | - |
Cavia porcellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
Ala-Ala-Pro-Phe-4-nitroanilide | wild type enzyme | Cavia porcellus | |
0.098 | - |
Ala-Ala-Pro-Met-4-nitroanilide | wild type enzyme | Cavia porcellus | |
0.93 | - |
Ala-Ala-Pro-Leu-4-nitroanilide | wild type enzyme | Cavia porcellus | |
3.9 | - |
Ala-Ala-Pro-Leu-4-nitroanilide | mutant enzyme A216G | Cavia porcellus | |
13 | - |
Ala-Ala-Pro-Met-4-nitroanilide | mutant enzyme A216G | Cavia porcellus | |
16 | - |
Ala-Ala-Pro-Phe-4-nitroanilide | mutant enzyme A216G | Cavia porcellus | |
19.3 | - |
Ala-Ala-Pro-Leu-4-nitroanilide | wild type enzyme | Homo sapiens | |
23 | - |
Ala-Ala-Pro-Met-4-nitroanilide | wild type enzyme | Homo sapiens | |
143 | - |
Ala-Ala-Pro-Phe-4-nitroanilide | wild type enzyme | Homo sapiens |