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Literature summary for 3.4.21.39 extracted from

  • Ferry, G.; Gillet, L.; Bruneau, V.; Banales, J.M.; Beauverger, P.; Coge, F.; Galizzi, J.P.; Scalbert, E.; Okamoto, T.; Urata, H.; Boutin, J.A.
    Development of new assays and improved procedures for the purification of recombinant human chymase (2001), Eur. J. Biochem., 268, 5885-5893.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
produced in CHO cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin
-
Homo sapiens
chymostatin
-
Homo sapiens
dipyridyl IC50: above 0.4 mM Homo sapiens
lisinopril IC50: above 0.4 mM Homo sapiens
SD906
-
Homo sapiens
soybean tryosin inhibitor
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens chymase mediates a major alternative way of angiotensin II production from angiotensin I beside angiotensin converting enzyme in the final step of the renin-angiotensin system. This enzyme is also involved in other physio-pathological processes such as angiogenesis, atherosclerosis and inflammation ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is produced as inactive prochymase Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.16
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Dab(DNP) + H2O the enzyme cleaves between Phe and His of modified angiotensin I permitting the release of His-[2-amino-4-(2,3-dinitrophenylamino)-butyrate] Homo sapiens 2-aminobenzoyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + His-Dab(DNP)
-
?
additional information chymase mediates a major alternative way of angiotensin II production from angiotensin I beside angiotensin converting enzyme in the final step of the renin-angiotensin system. This enzyme is also involved in other physio-pathological processes such as angiogenesis, atherosclerosis and inflammation Homo sapiens ?
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.4
-
IC50: above 0.4 mM Homo sapiens lisinopril
0.4
-
IC50: above 0.4 mM Homo sapiens dipyridyl