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Literature summary for 3.4.21.37 extracted from

  • Kurtagic, E.; Jedrychowski, M.P.; Nugent, M.A.
    Neutrophil elastase cleaves VEGF to generate a VEGF fragment with altered activity (2009), Am. J. Physiol. Lung Cell Mol. Physiol., 296, L534-L546.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.37

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
 Sprague-Dawley rats
-

Source Tissue

Source Tissue Comment Organism Textmining
aorta aortic endothelial cells Bos taurus
-
aorta aortic smooth muscle cells Rattus norvegicus
-
artery pulmonary artery endothelial cells Bos taurus
-
endothelial cell pulmonary artery endothelial cells and aortic endothelial cells Bos taurus
-
lung pulmonary artery endothelial cells Bos taurus
-
lung fibroblast
-
 Rattus norvegicus
-
macrophage
-
 Mus musculus
-
monocyte
-
 Mus musculus
-
RAW-264.7 cell
-
 Mus musculus
-
smooth muscle cell neonatal, aortic Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information neutrophil elastase cleaves vascular endothelial growth factor, VEGF, to generate a VEGF fragment with altered activity. Recombinant human VEGF165 is a substrate for the enzyme. The neutrophil elastase-generated VEGF fragment shows significantly reduced binding to VEGF receptor 2 and heparin, yet retains the ability to bind to VEGF receptor 1. Substrate and product analysis by mass spectrometry Rattus norvegicus ?
-
 ?
additional information neutrophil elastase cleaves vascular endothelial growth factor, VEGF, to generate a VEGF fragment with altered activity. Recombinant human VEGF165 is a substrate for the enzyme. The neutrophil elastase-generated VEGF fragment shows significantly reduced binding to VEGF receptor 2 and heparin, yet retains the ability to bind to VEGF receptor 1. The VEGF fragment shows altered signaling in pulmonary artery endothelial cells compared with intact VEGF165, detailed overview. Substrate and product analysis by mass spectrometry. PDGF is also a substrate of neutrophil elastase, while TNFalpha and FGF2 are not Bos taurus ?
-
 ?
additional information neutrophil elastase cleaves vascular endothelial growth factor, VEGF, to generate a VEGF fragment with altered activity. Recombinant human VEGF165 is a substrate for the enzyme. The neutrophil elastase-generated VEGF fragment, VEGFf, and the wild-type VEGF both activate Akt, and VEGFf appears to be more effective. Substrate and product analysis by mass spectrometry Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
neutrophil elastase
-
 Mus musculus
neutrophil elastase
-
 Rattus norvegicus
neutrophil elastase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Bos taurus
37
-
 cell-based assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus
7.4
-
 assay at Bos taurus
7.4
-
 cell-based assay at Rattus norvegicus

General Information

General Information Comment Organism
malfunction excessive neutrophil elastase activity and altered vascular endothelial growth factor signaling are independently in the development and progression of pulmonary emphysema Mus musculus
malfunction excessive neutrophil elastase activity and altered vascular endothelial growth factor signaling are independently in the development and progression of pulmonary emphysema Rattus norvegicus
malfunction excessive neutrophil elastase activity and altered vascular endothelial growth factor signaling are independently in the development and progression of pulmonary emphysema Bos taurus