Cloned (Comment) | Organism |
---|---|
expressed in RBL-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A213V | the mutant shows increased kcat and Km values compared to the wild type enzyme | Homo sapiens |
F192A | the mutant shows increased kcat and Km values compared to the wild type enzyme | Homo sapiens |
F192K | the mutant shows increased kcat and Km values compared to the wild type enzyme | Homo sapiens |
F215A | very low activity, the mutant shows decreased kcat and increased Km values compared to the wild type enzyme | Homo sapiens |
F215Y | the mutant shows decreased kcat and Km values compared to the wild type enzyme | Homo sapiens |
N98T | the mutant shows increased kcat and Km values compared to the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-methoxysuccinyl-Ala-Ala-Pro-Val chloromethyl ketone | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F215Y, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
0.07 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | wild type enzyme, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
0.09 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme N98T, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
0.79 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F192K, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
1.04 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F215A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
1.17 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme A213V, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
5.02 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F192A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
specific granule | neutrophil granule | Homo sapiens | 42581 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IpaB protein + H2O | Homo sapiens | neutrophil elastase cleaves IpaB protein after alanine, valine, isoleucine, or threonine residues | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neutrophil | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IpaB protein + H2O | neutrophil elastase cleaves IpaB protein after alanine, valine, isoleucine, or threonine residues | Homo sapiens | ? | - |
? | |
additional information | does not cleave N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | Homo sapiens | ? | - |
? | |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O | specific neutrophil elastase substrate | Homo sapiens | N-methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutrophil elastase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.42 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F215A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
5.48 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F215Y, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
6.71 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | wild type enzyme, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
8.05 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme A213V, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
10.7 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F192A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
11.63 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme F192K, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens | |
63.11 | - |
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | mutant enzyme N98T, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C | Homo sapiens |