Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.36 extracted from

  • Sadeghi-Kaji, S.; Shareghi, B.; Saboury, A.A.; Farhadian, S.
    Spectroscopic and molecular docking studies on the interaction between spermidine and pancreatic elastase (2019), Int. J. Biol. Macromol., 131, 473-483 .
    View publication on PubMed
Search for more literature for 3.4.21.36

Activating Compound

Activating Compound Comment Organism Structure
spermidine spermidine serves as a partial stabilizer and an activator for the enzyme Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07761
-
 4-nitrophenyl acetate in the absence of spermidine, at pH 8.5 and 37°C Sus scrofa
0.08229
-
 4-nitrophenyl acetate in the presence of 0.2 mM spermidine, at pH 8.5 and 37°C Sus scrofa
0.09364
-
 4-nitrophenyl acetate in the presence of 0.3 mM spermidine, at pH 8.5 and 37°C Sus scrofa
0.11568
-
 4-nitrophenyl acetate in the presence of 0.5 mM spermidine, at pH 8.5 and 37°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Sus scrofa 4-nitrophenol + acetate
-
 ?

Synonyms

Synonyms Comment Organism
pancreatic elastase
-
 Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 melting temperature Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0058
-
 4-nitrophenyl acetate in the absence of spermidine, at pH 8.5 and 37°C Sus scrofa
0.007
-
 4-nitrophenyl acetate in the presence of 0.2 mM spermidine, at pH 8.5 and 37°C Sus scrofa
0.0092
-
 4-nitrophenyl acetate in the presence of 0.3 mM spermidine, at pH 8.5 and 37°C Sus scrofa
0.0125
-
 4-nitrophenyl acetate in the presence of 0.5 mM spermidine, at pH 8.5 and 37°C Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Sus scrofa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.075
-
 4-nitrophenyl acetate in the absence of spermidine, at pH 8.5 and 37°C Sus scrofa
0.085
-
 4-nitrophenyl acetate in the presence of 0.2 mM spermidine, at pH 8.5 and 37°C Sus scrofa
0.097
-
 4-nitrophenyl acetate in the presence of 0.3 mM spermidine, at pH 8.5 and 37°C Sus scrofa
0.107
-
 4-nitrophenyl acetate in the presence of 0.5 mM spermidine, at pH 8.5 and 37°C Sus scrofa