Literature summary for 3.4.21.34 extracted from

Tada, M.; Tsuda, Y.; Wanaka, K.; Hijikata-Okunomiya, A.; Horie, N.; Okamoto, U.; Okamoto, S.; Okada, Y.
Isolation of plasma kallikrein by high efficiency affinity chromatography and its characterization (2001), Biol. Pharm. Bull., 24, 520-524.

Search for more literature for 3.4.21.34

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	plasma	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
86150	-	MALDI TOF mass spectroscopy	Homo sapiens
88000	-	1 * 88000, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
kininogen + H2O	Homo sapiens	-	bradykinin + fragment of kininogen	-	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
466fold from plasma, affinity chromatography with inhibitor PKSI-527, i.e. trans-4-aminomethylcyclohexanecarbonyl-L-phenylalanyl-4-aminophenyl acetic acid, as ligand	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
plasma	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
28.9	-	purified enzyme, substrate D-Pro-Phe-Arg-4-nitroanilide	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Pro-Phe-Arg-4-nitroanilide + H2O	synthetic fluorogenic substrate	Homo sapiens	D-Pro-Phe-Arg + 4-nitroaniline	-	?
kininogen + H2O	-	Homo sapiens	bradykinin + fragment of kininogen	-	?
kininogen + H2O	-	Homo sapiens	bradykinin + fragment of kininogen	-	ir

Subunits

Subunits	Comment	Organism
monomer	1 * 88000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PK	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)