Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.32 extracted from

  • Sakharov, I.Y.; Litvin, F.E
    Artyukov, A.A.: Purification and characterization of two serine collagenolytic proteases from crab Paralithodes camtschatica (1994), Comp. Biochem. Physiol. B, 108, 561-568.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
basic pancreatic trypsin inhibitor
-
Paralithodes camtschaticus
diisopropylfluorophosphate
-
Paralithodes camtschaticus
Phenylmethylsulfonylfluoride
-
Paralithodes camtschaticus
Soybean trypsin inhibitor
-
Paralithodes camtschaticus
tosyl-Lys-CH2Cl protease A Paralithodes camtschaticus
tosyl-Phe-CH2Cl protease C Paralithodes camtschaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000, SDS-PAGE, protease C Paralithodes camtschaticus
30000
-
x * 30000, SDS-PAGE, protease A Paralithodes camtschaticus

Organism

Organism UniProt Comment Textmining
Paralithodes camtschaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protease A and C Paralithodes camtschaticus

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
Paralithodes camtschaticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.4
-
protease C Paralithodes camtschaticus
1330
-
protease A Paralithodes camtschaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O
-
Paralithodes camtschaticus Hydrolyzed collagen
-
?
N-benzoyl-L-Arg-p-nitroanilide + H2O
-
Paralithodes camtschaticus ?
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
-
Paralithodes camtschaticus ?
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE, protease A Paralithodes camtschaticus
? x * 24000, SDS-PAGE, protease C Paralithodes camtschaticus