Literature summary for 3.4.21.27 extracted from

Smith, S.B.; Verhamme, I.M.; Sun, M.F.; Bock, P.E.; Gailani, D.
Characterization of novel forms of coagulation factor XIa. Independence of factor XIa subunits in factor IX activation (2008), J. Biol. Chem., 283, 6696-6705.

Search for more literature for 3.4.21.27

Cloned(Commentary)

Cloned (Comment)	Organism
stable expression of wild-type and mutant enzymes in HEK-293 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C362S/C482S	site-directed mutagenesis	Homo sapiens
additional information	construction of FXI with a single catalytic active site, construction of recombinant FXI with the A4 domain replaced by the A4 domain from plasma prekallikrein, i.e. FXI/PKA4, overview	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
D-Phe-Pro-Arg-CH2Cl	irreversibly inhibits both active sites of the enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics of isolated subunits with each one active site, stoichiometry of FIX and FIXalphabeta binding to FXIa, overview	Homo sapiens
0.053	-	factor IX	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.4	-	pyroGlu-Pro-Arg-4-nitroanilide	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Homo sapiens	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
factor IX + H2O	Homo sapiens	two forms of activated factor XI are generated during coagulation, and each half of a factor XIa dimer behaves as an independent enzyme with respect to factor IX	activated factor IX + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	FXI activation proceeds through an intermediate with one activated subunit, limited digestion by incubating with FXIIa or thrombin	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from plasma and recombinant wild-type and mutant enzymes from HEK-293 cells by immunoaffinity chromatography with elution by benzamidine	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Homo sapiens	-
plasma	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	cleavage at Ala145 and Ala180, activity of wild-type and mutant enzymes with wild-type andmutant substrate, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
factor IX + H2O	two forms of activated factor XI are generated during coagulation, and each half of a factor XIa dimer behaves as an independent enzyme with respect to factor IX	Homo sapiens	activated factor IX + ?	-	?
factor IX + H2O	cleavage at Ala145 and Ala180, activity of wild-type and mutant enzymes with wild-type and mutant substrate, overview	Homo sapiens	activated factor IX + ?	-	?
methyl-sulfonyl-D-cyclo-hexyl-glycyl-glycyl-arginine-p-nitroanilide + H2O	substrate S-299	Homo sapiens	?	-	?
pyroGlu-Pro-Arg-4-nitroanilide + H2O	substrate S-2366	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
dimer	two forms of activated factor XI are generated during coagulation, and each half of a factor XIa dimer behaves as an independent enzyme with respect to factor IX	Homo sapiens

Synonyms

Synonyms	Comment	Organism
FXIa	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.268	-	pyroGlu-Pro-Arg-4-nitroanilide	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.55	-	factor IX	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	-	Homo sapiens

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Release 2023.1 (January 2023)