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Literature summary for 3.4.21.27 extracted from
- A catalytic domain exosite (Cys527-Cys542) in factor XIa mediates binding to a site on activated platelets (2007), Biochemistry, 46, 14450-14460.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant enzyme C362S/C482S in HEK-293 cells and secretion of recombinant protein to the medium | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C362S/C482S | site-directed mutagenesis, the mutant shows increased platelet binding compared to the wild-type enzyme | Homo sapiens |
| additional information | chimeric recombinant enzyme mutants rFXI-PKA3 and rFXIa-PKA3, with fusion of FXI to the Apple 3 domain of prekallikrein, show unaffected platelet binding compared to the wild-type enzyme, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the recombinant catalytic domain Ile370-Val607 of FXIa inhibits the binding of factor XIa to the platelets with a Ki of 3.5 nM, whereas the recombinant factor XI heavy chain does not | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
2 * 80000, recombinant mutant C362S/C482S , SDS-PAGE | Homo sapiens |
| 160000 | - |
recombinant mutant C362S/C482S , native PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor IX + H2O | Homo sapiens | - |
activated factor IX + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P03951 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | factor XI is activated to factor XIIa at 37°C, a conformational transition accompanies conversion of factor XI to factor XIa that conceals the Apple 3 domain zymogen factor XI platelet binding site and exposes the factor XIa platelet binding site within the catalytic domain possibly comprising residues Cys527-Cys542 | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant mutant enzyme C362S/C482S from HEK-293 cells by immunoaffinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | from plasma | Homo sapiens | - |
| additional information | the enzyme binds to platelets, a conformational transition accompanies conversion of factor XI to factor XIa that conceals the Apple 3 domain zymogen factor XI platelet binding site and exposes the factor XIa platelet binding site within the catalytic domain possibly comprising residues Cys527-Cys542, overview | Homo sapiens | - |
| plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor IX + H2O | - |
Homo sapiens | activated factor IX + ? | - |
? | |
| additional information | a conformational transition accompanies conversion of factor XI to factor XIa that conceals the Apple 3 domain zymogen factor XI platelet binding site and exposes the factor XIa platelet binding site within the catalytic domain possibly comprising residues Cys527-Cys542 | Homo sapiens | ? | - |
? | |
| S-2366 + H2O | commercial chromogenic substrate | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 80000, recombinant mutant C362S/C482S , SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| factor XIa | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | competition for platelet binding by the wild-type enzyme and mutants, overview | Homo sapiens |
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