Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in HEK-293 cells and secretion of recombinant protein to the medium | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K145A | site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme | Homo sapiens |
K148A | site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme | Homo sapiens |
K149A | site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme | Homo sapiens |
R144A | site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme | Homo sapiens |
R144A/K145A/R147A/K148A/K149A | site-directed mutagenesis of an autolysis loop residues, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme | Homo sapiens |
R147A | site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha1-protease inhibitor | - |
Homo sapiens | |
antithrombin | - |
Homo sapiens | |
C1-inhibitor | - |
Homo sapiens | |
protein Z dependent protease inhibitor | - |
Homo sapiens | |
serpins | inhibition of autolysis of fXIa | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor IX + H2O | Homo sapiens | - |
activated factor IX + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P03951 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme shows activation by autolysis involving residues of the autolysis loops, overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from HEK-293 cells by immunoaffinity, cation exchange, and heparin affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor IX + H2O | - |
Homo sapiens | activated factor IX + ? | - |
? | |
S-2366 + H2O | commercial chromogenic substrate | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor XIa | - |
Homo sapiens |
FXIa | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | second-order inhibition rate constants, wild-type and mutant enzymes with different inhibitors, overview | Homo sapiens |