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Literature summary for 3.4.21.27 extracted from

  • Rezaie, A.R.; Sun, M.F.; Gailani, D.
    Contributions of basic amino acids in the autolysis loop of factor XIa to serpin specificity (2006), Biochemistry, 45, 9427-9433.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in HEK-293 cells and secretion of recombinant protein to the medium Homo sapiens

Protein Variants

Protein Variants Comment Organism
K145A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
K148A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
K149A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
R144A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
R144A/K145A/R147A/K148A/K149A site-directed mutagenesis of an autolysis loop residues, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
R147A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha1-protease inhibitor
-
Homo sapiens
antithrombin
-
Homo sapiens
C1-inhibitor
-
Homo sapiens
protein Z dependent protease inhibitor
-
Homo sapiens
serpins inhibition of autolysis of fXIa Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor IX + H2O Homo sapiens
-
activated factor IX + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P03951
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme shows activation by autolysis involving residues of the autolysis loops, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from HEK-293 cells by immunoaffinity, cation exchange, and heparin affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor IX + H2O
-
Homo sapiens activated factor IX + ?
-
?
S-2366 + H2O commercial chromogenic substrate Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
factor XIa
-
Homo sapiens
FXIa
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information second-order inhibition rate constants, wild-type and mutant enzymes with different inhibitors, overview Homo sapiens