Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.27 extracted from

  • Gailani, D.; Ho, D.; Sun, M.F.; Cheng, Q.; Walsh, P.N.
    Model for a factor IX activation complex on blood platelets: dimeric conformation of factor XIa is essential (2001), Blood, 97, 3117-3122.
    View publication on PubMed
Search for more literature for 3.4.21.27

Protein Variants

Protein Variants Comment Organism
additional information FXIa/PKA4 is made by replacing the FXI A4 domain with the A4 domain from prekallikrein. A dimeric version of FXI/PKA4 (FXI/PKA4-Gly326) is prepared as a control. Activated FXIa/PKA4 and FXIa/PKA4-Gly326 activate factor IX with kinetic parameters similar to that of FXIa. FXI/PKA4 and FXIa/PKA4-Gly326 have coagulant activity similar to FXI. FXIa/PKA4, FXIa/PKA4-Gly326 and FXIa have similar affinities for activated platelets. The dimeric proteins FXI and FXI/PKA4-Gly326 promote coagulation similarly, however, monomeric FXIa/PKA4 has greatly reduced activity. The activated monomeric FXIa/PKA4 activates factor IX poorly in the presence of activated platelets Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
 factor IX FXIa/PKA4, 37°C Homo sapiens
0.00016
-
 factor IX FXIa/PKA4-Gly326, 37°C Homo sapiens
0.0002
-
 factor IX factor FXIa, 37°C Homo sapiens
0.45
-
 L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4 Homo sapiens
0.5
-
 L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide factor FXIa Homo sapiens
0.59
-
 L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4-Gly326 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor IX + H2O model for factor IX activation in which FXIa binds to activated platelets by one chain of the dimer, while binding to factor IX through the other Homo sapiens activated factor IX + ?
-
 ?
L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens L-pyroglutamyl-L-Pro-L-Arg + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
FXIa
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
 factor IX FXIa/PKA4, 37°C Homo sapiens
5.7
-
 factor IX FXIa/PKA4-Gly326, 37°C Homo sapiens
5.8
-
 factor IX factor FXIa, 37°C Homo sapiens
250
-
 L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4 Homo sapiens
310
-
 L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide factor FXIa Homo sapiens
430
-
 L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4-Gly326 Homo sapiens