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Literature summary for 3.4.21.26 extracted from
- Identification and characterization of a novel prolyl oligopeptidase in filarial parasite Setaria cervi (2018), Biochem. Biophys. Res. Commun., 495, 2235-2241 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | about 59% inhibition at 10 mM | Setaria cervi |  |
| 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride | complete inhibition at 10 mM | Setaria cervi | |
| 5,5'-dithio-bis-(2-nitrobenzoic acid) | complete inhibition at 5 mM | Setaria cervi | |
| berberine chloride | - |
Setaria cervi | |
| iodoacetamide | 23.61% residual activity at 10 mM | Setaria cervi | |
| N-ethylmaleimide | complete inhibition at 10 mM | Setaria cervi | |
| rosmarinic acid | - |
Setaria cervi | |
| Z-Pro-prolinal | specific inhibitor | Setaria cervi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0308 | - |
Z-Gly-Pro-4-nitroanilide | at pH 8.0 and 37°C | Setaria cervi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Setaria cervi | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Setaria cervi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation DEAE Sepharose column chromatography, and phenyl Sepharose column chromatography | Setaria cervi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.097 | - |
crude cytosolic extract, at pH 8.0 and 37°C | Setaria cervi |
| 225.93 | - |
after 44.326fold purification, at pH 8.0 and 37°C | Setaria cervi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Z-Gly-Pro-4-nitroanilide + H2O | - |
Setaria cervi | Z-Gly-Pro + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, SDS-PAGE | Setaria cervi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| POP | - |
Setaria cervi |
| prolyl endopeptidase | - |
Setaria cervi |
| prolyl oligopeptidase | - |
Setaria cervi |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00000287 | - |
at pH and °C | Setaria cervi | Z-Pro-prolinal | |
| 0.0524 | - |
at pH 8.0 and 37°C | Setaria cervi | rosmarinic acid | |
| 0.128 | - |
at pH 8.0 and 37°C | Setaria cervi | berberine chloride | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | enzyme inhibition induces calcium signaling via phospholipase c stimulation which further triggers mitochondrial mediated apoptosis in filarial parasites | Setaria cervi |
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