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Literature summary for 3.4.21.26 extracted from

  • Harris, M.N.; Madura, J.D.; Ming, L.J.; Harwood, V.J.
    Kinetic and mechanistic studies of prolyl oligopeptidase from the hyperthermophile Pyrococcus furiosus (2001), J. Biol. Chem., 276, 19310-19317.
    View publication on PubMed
Search for more literature for 3.4.21.26

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)-benzenesulfonyl fluoride
-
 Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic and mechanistic studies Pyrococcus furiosus
0.0194
-
 Ala-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.0207
-
 Gly-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.0229
-
 Arg-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.04
-
 Asp-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.043
-
 benzyloxycarbonyl-Ala-Ala-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.0437
-
 benzyloxycarbonyl-Ala-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.16
-
 Arg-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
NaBr activates. There are five non-productive binding sites for Cl-, and two for F- and Br-, suggesting that Cl- must bind differently from F- and Br- Pyrococcus furiosus
NaCl activates. There are five non-productive binding sites for Cl-, and two for F- and Br-, suggesting that Cl- must bind differently from F- and Br- Pyrococcus furiosus
NaF activates. There are five non-productive binding sites for Cl-, and two for F- and Br-, suggesting that Cl- must bind differently from F- and Br- Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Pro-4-nitroanilide + H2O
-
 Pyrococcus furiosus Ala-Pro + 4-nitroaniline
-
 ?
Arg-Pro-4-nitroanilide + H2O
-
 Pyrococcus furiosus Arg-Pro + 4-nitroaniline
-
 ?
Asp-Pro-4-nitroanilide + H2O
-
 Pyrococcus furiosus Asp-Pro + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Ala-4-nitroanilide + H2O
-
 Pyrococcus furiosus benzyloxycarbonyl-Ala-Ala + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Pro-4-nitroanilide + H2O
-
 Pyrococcus furiosus benzyloxycarbonyl-Ala-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Pyrococcus furiosus Gly-Pro + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kinetic and mechanistic studies Pyrococcus furiosus
0.03
-
 Arg-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.06
-
 Gly-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.073
-
 Asp-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
0.079
-
 Ala-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
2.5
-
 benzyloxycarbonyl-Ala-Ala-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
16.9
-
 Arg-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
17.9
-
 benzyloxycarbonyl-Ala-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 doubly sigmoidal pH-activity profiles Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
 4-(2-aminoethyl)-benzenesulfonyl fluoride pH 7.5, 85°C Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the enzyme is implicated in neuropeptide processing Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information kinetic and mechanistic studies Pyrococcus furiosus
1.31
-
 Arg-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
1.82
-
 Asp-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
2.9
-
 Gly-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
4.08
-
 Ala-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
57.6
-
 benzyloxycarbonyl-Ala-Ala-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
106
-
 Arg-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus
410
-
 benzyloxycarbonyl-Ala-Pro-4-nitroanilide pH 7.5, 85°C Pyrococcus furiosus