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Literature summary for 3.4.21.21 extracted from

  • Olsen, O.H.; Nielsen, P.F.; Persson, E.
    Prevention of beta strand movement into a zymogen-like position does not confer higher activity to coagulation factor VIIa (2004), Biochemistry, 43, 14096-14103.
    View publication on PubMed
Search for more literature for 3.4.21.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baby hamster kidney cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C164V/V299C-FVIIa introduction of a new disulfide bridge between Cys-159 and an introduced Cys at position 299 Homo sapiens
L280I/V299M-FVIIa without description Homo sapiens
V299M-FVIIa modification of the first Leu-X-Val motif by the introduction of Met in the third position Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide wild type enzyme, in the presence of tissue factor, pH 7.4 Homo sapiens
2
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide mutant enzyme L280I/V299M-FVIIa, in the presence of tissue factor, pH 7.4 Homo sapiens
2.2
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide mutant enzyme C164V/V299C FVIIa, in the presence of tissue factor, pH 7.4 Homo sapiens
2.3
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide mutant enzyme V299M-FVIIa, in the presence of tissue factor, pH 7.4 Homo sapiens
6.6
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide mutant enzyme L280I/V299M-FVIIa, pH 7.4 Homo sapiens
8
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide wild type enzyme, pH 7.4 Homo sapiens
9.2
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide mutant enzyme C164V/V299C, pH 7.4 Homo sapiens
9.8
-
 H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide mutant enzyme V299M-FVIIa, pH 7.4 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
method not mentioned Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
 Homo sapiens factor Xa
-
 ?
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide + H2O i.e. S-2288 Homo sapiens H-D-isoleucyl-L-prolyl-arginine + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
factor VIIa
-
 Homo sapiens