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Literature summary for 3.4.21.21 extracted from

  • Toso, R.; Pinotti, M.; High, K.A.; Pollak, E.S.; Bernardi, F.
    A frequent human coagulation Factor VII mutation (A294V, c152) in loop 140s affects the interaction with activators, tissue factor and substrates (2002), Biochem. J., 363, 411-416.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
tissue factor the enzyme initiates blood clotting after interacting with its cofactor tissue factor. Soluble tissue factor concentrations of 5.0 nM and 22.4 nM for the activated wild-type factor VII and for the mutant form A294V of the activated wild-type factor yield an activity of 50% towards spectrozyme fVIIa Homo sapiens

Protein Variants

Protein Variants Comment Organism
A294V mutant enzyme shows delayed activation by activated factor X as well as reduced activity towards peptidyl and macromolecular substrates without impairing the catalytic efficiency of the triad Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the KM-value for spectrozyme fVIIa for the mutant enzyme A294V is above 2.5 mM Homo sapiens
0.67
-
 spectrozyme fVIIa wild-type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor X + H2O Homo sapiens the enzyme initiates blood clotting after interacting with its cofactor tissue factor. The product of the reaction, activated factor X, is also the most efficient activator of zymogen factor VII activated factor X + ?
-
 ?
spectrozyme fVIIa + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the product of the reaction with factor X, the activated factor X is also the most efficient activator of zymogen factor VII Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O the enzyme initiates blood clotting after interacting with its cofactor tissue factor. The product of the reaction, activated factor X, is also the most efficient activator of zymogen factor VII Homo sapiens activated factor X + ?
-
 ?
spectrozyme fVIIa + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
FVIIa
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 spectrozyme fVIIa mutant enzyme A294V Homo sapiens
17
-
 spectrozyme fVIIa wild-type enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
vitamin K dependent on Homo sapiens