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Literature summary for 3.4.21.117 extracted from

  • Fernandez, I.S.; Staendker, L.; Maegert, H.J.; Forssmann, W.G.; Gimenez-Gallego, G.; Romero, A.
    Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6) (2008), J. Mol. Biol., 377, 1488-1497.
    View publication on PubMed
Search for more literature for 3.4.21.117

Cloned(Commentary)

Cloned (Comment) Organism
gene KLK7, expression in Escherichia coli with secretion to the the bacterial periplasm Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme bound to specific inhibitor LEKTI domain 6, 8 mg/ml protein in sodium phosphate buffer, pH 7.5, with a reservoir solution containing 3.2 M ammonium sulfate in 0.1 M HEPES, pH 7.0, X-ray diffraction structure determination and anaylsis at 2.8 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
LEKTI domain 6 i.e. lympho-epithelial Kazaltype-related inhibitor domain 6, specific inhibition, interaction and binding structure analysis, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0159
-
 Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P49862 gene KLK7
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
epidermis
-
 Homo sapiens
-
lung adenocarcinoma cell
-
 Homo sapiens
-
lymphoblastic leukemia cell
-
 Homo sapiens
-
ovary
-
 Homo sapiens
-
pancreatic cancer cell
-
 Homo sapiens
-
skin kallikrein 7 is the major protease in human skin Homo sapiens
-
testicular carcinoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More three-dimensional structure and specific substrate site, involving conserved residues Ala190-Ser195, Val213-Cys220 and Pro225-Tyr228, molecular modeling, overview Homo sapiens

Synonyms

Synonyms Comment Organism
kallikrein 7
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.026
-
-
 Homo sapiens LEKTI domain 6