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Literature summary for 3.4.21.117 extracted from

  • Ramani, V.C.; Haun, R.S.
    The extracellular matrix protein fibronectin is a substrate for kallikrein 7 (2008), Biochem. Biophys. Res. Commun., 369, 1169-1173.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene KLK7, stable expression of pro-kallikrein 7 in HEK-293F cells, secretion of the recombinant protein to the cell culture supernatant, activation of the recombinant enzyme by thermolysin Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
chymostatin
-
Homo sapiens
additional information phosphoramidon inhibits thermolysin and thus activation of pro-kallikrein 7 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibronectin + H2O Homo sapiens kallikrein degrades components of the extracellular matrix ?
-
?
additional information Homo sapiens kallikrein 7 participates in normal desquamation by facilitating cell shedding at the skin surface, and it degrades components of the extracellular matrix, the aberrant expression and secretion of kallikrein 7 in human tumors may facilitate metastasis by directly degrading components of the extracellular matrix and may thus play an important role in tumorigenesis, overview, the enzyme is implicated in the degradation of intercellular cohesive structures in the stratum corneum preceding desquamation ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene KLK7
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation by thermolysin, inhibited by phosphoramidon Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant kallikrein 7 from HEK-293F cell culture supernatant by ultrafiltration and dialysis, anion exchange chromatography, and heparin affinity chromatography, followed by metal affinity on an iminodiacetic acid epoxy resin column, elution with L-histidine from the latter column, to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreatic cancer cell kallikrein 7 is overexpressed in pancreatic adenocarcinomas as well as other human cancers Homo sapiens
-
stratum corneum
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fibronectin + H2O kallikrein degrades components of the extracellular matrix Homo sapiens ?
-
?
Fibronectin + H2O kallikrein 7 is a chymostatin-like serine protease Homo sapiens ?
-
?
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O a fluorogenic peptide substrate Homo sapiens ?
-
?
additional information kallikrein 7 participates in normal desquamation by facilitating cell shedding at the skin surface, and it degrades components of the extracellular matrix, the aberrant expression and secretion of kallikrein 7 in human tumors may facilitate metastasis by directly degrading components of the extracellular matrix and may thus play an important role in tumorigenesis, overview, the enzyme is implicated in the degradation of intercellular cohesive structures in the stratum corneum preceding desquamation Homo sapiens ?
-
?
additional information no activity with laminin Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
kallikrein 7
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens