Cloned (Comment) | Organism |
---|---|
gene KLK7, stable expression of pro-kallikrein 7 in HEK-293F cells, secretion of the recombinant protein to the cell culture supernatant, activation of the recombinant enzyme by thermolysin | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymostatin | - |
Homo sapiens | |
additional information | phosphoramidon inhibits thermolysin and thus activation of pro-kallikrein 7 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibronectin + H2O | Homo sapiens | kallikrein degrades components of the extracellular matrix | ? | - |
? | |
additional information | Homo sapiens | kallikrein 7 participates in normal desquamation by facilitating cell shedding at the skin surface, and it degrades components of the extracellular matrix, the aberrant expression and secretion of kallikrein 7 in human tumors may facilitate metastasis by directly degrading components of the extracellular matrix and may thus play an important role in tumorigenesis, overview, the enzyme is implicated in the degradation of intercellular cohesive structures in the stratum corneum preceding desquamation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene KLK7 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | activation by thermolysin, inhibited by phosphoramidon | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant kallikrein 7 from HEK-293F cell culture supernatant by ultrafiltration and dialysis, anion exchange chromatography, and heparin affinity chromatography, followed by metal affinity on an iminodiacetic acid epoxy resin column, elution with L-histidine from the latter column, to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreatic cancer cell | kallikrein 7 is overexpressed in pancreatic adenocarcinomas as well as other human cancers | Homo sapiens | - |
stratum corneum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibronectin + H2O | kallikrein degrades components of the extracellular matrix | Homo sapiens | ? | - |
? | |
Fibronectin + H2O | kallikrein 7 is a chymostatin-like serine protease | Homo sapiens | ? | - |
? | |
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O | a fluorogenic peptide substrate | Homo sapiens | ? | - |
? | |
additional information | kallikrein 7 participates in normal desquamation by facilitating cell shedding at the skin surface, and it degrades components of the extracellular matrix, the aberrant expression and secretion of kallikrein 7 in human tumors may facilitate metastasis by directly degrading components of the extracellular matrix and may thus play an important role in tumorigenesis, overview, the enzyme is implicated in the degradation of intercellular cohesive structures in the stratum corneum preceding desquamation | Homo sapiens | ? | - |
? | |
additional information | no activity with laminin | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
kallikrein 7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |