Cloned (Comment) | Organism |
---|---|
gene KLK7, encoded on chromosome 19, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)SS with secretion to the the bacterial periplasm | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting-drop vapour-diffusion method, 22°C, 0.0001 ml of 8 mg/ml protein in 50 mM sodium phosphate buffer, pH 7.5, mixed with 0.0001 ml of reservoir solution, X-ray diffraction structure determination and anaylsis at 2.8 A resolution | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | kallikrein 7 is involved in pathological keratinization, psoriasis and ovarian cancer | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)SS periplasm by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | kallikrein 7 is involved in pathological keratinization, psoriasis and ovarian cancer | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular structure of kallikrein 7 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
kallikrein 7 | - |
Homo sapiens |
More | the enzyme belongs to the multigene kallikrein serine protease family | Homo sapiens |