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Literature summary for 3.4.21.116 extracted from

  • Rudner, D.Z.; Fawcett, P.; Losick, R.
    A family of membrane-embedded metalloproteases involved in regulated proteolysis of membrane-associated transcription factors (1999), Proc. Natl. Acad. Sci. USA, 96, 14765-14770.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and enzyme mutants in mutants strain BSL51 and R13 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D137A site-directed mutagenesis, mutant is completely impaired in pro-sigmaK processing Bacillus subtilis
D137E site-directed mutagenesis, mutant is completely impaired in pro-sigmaK processing Bacillus subtilis
D137N site-directed mutagenesis, mutant is completely impaired in pro-sigmaK processing Bacillus subtilis
E44A site-directed mutagenesis, no activity Bacillus subtilis
E44D site-directed mutagenesis, unaltered activity, similar to wild-type Bacillus subtilis
E44Q site-directed mutagenesis, no activity Bacillus subtilis
G138A site-directed mutagenesis, slightly reduced activity Bacillus subtilis
G139A site-directed mutagenesis, slightly reduced activity Bacillus subtilis
H43A site-directed mutagenesis, no activity Bacillus subtilis
H43F site-directed mutagenesis, extremely low enzyme expression, no activity Bacillus subtilis
H47F site-directed mutagenesis, no activity Bacillus subtilis
N122A site-directed mutagenesis, extremely low enzyme expression Bacillus subtilis
N122D site-directed mutagenesis, extremely low enzyme expression Bacillus subtilis
N122Q site-directed mutagenesis, unaltered activity, similar to wild-type Bacillus subtilis
N129A site-directed mutagenesis, slightly reduced activity Bacillus subtilis
P132A site-directed mutagenesis, highly reduced enzyme activity Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane catalytic center is located adjacent tot or within the membrane Bacillus subtilis 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-sigmaK + H2O Bacillus subtilis processing of prosigmaK, which is a protein involved in sporulation sigmaK + 20 amino acid peptide
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?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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wild-type strain PY79
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Reaction

Reaction Comment Organism Reaction ID
self-cleaves Val52-/-Asn53, Ala62-/-Phe63 and Val74-/-Thr75 at the N-terminus of SpoIVB D137 is essential for catalytic activity, mechanism model, enzyme contains conserved metalloprotease sequence motifs HEXXH and NPDG, which are expected to form the catalytic center and are required for catalytic activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-sigmaK + H2O processing of prosigmaK, which is a protein involved in sporulation Bacillus subtilis sigmaK + 20 amino acid peptide
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?

Synonyms

Synonyms Comment Organism
M50.002 Merops-ID Bacillus subtilis
SpolVFB
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Bacillus subtilis
stage IV sporulation protein FB
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Bacillus subtilis