Cloned (Comment) | Organism |
---|---|
expression of wild-type and enzyme mutants in mutants strain BSL51 and R13 | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D137A | site-directed mutagenesis, mutant is completely impaired in pro-sigmaK processing | Bacillus subtilis |
D137E | site-directed mutagenesis, mutant is completely impaired in pro-sigmaK processing | Bacillus subtilis |
D137N | site-directed mutagenesis, mutant is completely impaired in pro-sigmaK processing | Bacillus subtilis |
E44A | site-directed mutagenesis, no activity | Bacillus subtilis |
E44D | site-directed mutagenesis, unaltered activity, similar to wild-type | Bacillus subtilis |
E44Q | site-directed mutagenesis, no activity | Bacillus subtilis |
G138A | site-directed mutagenesis, slightly reduced activity | Bacillus subtilis |
G139A | site-directed mutagenesis, slightly reduced activity | Bacillus subtilis |
H43A | site-directed mutagenesis, no activity | Bacillus subtilis |
H43F | site-directed mutagenesis, extremely low enzyme expression, no activity | Bacillus subtilis |
H47F | site-directed mutagenesis, no activity | Bacillus subtilis |
N122A | site-directed mutagenesis, extremely low enzyme expression | Bacillus subtilis |
N122D | site-directed mutagenesis, extremely low enzyme expression | Bacillus subtilis |
N122Q | site-directed mutagenesis, unaltered activity, similar to wild-type | Bacillus subtilis |
N129A | site-directed mutagenesis, slightly reduced activity | Bacillus subtilis |
P132A | site-directed mutagenesis, highly reduced enzyme activity | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | catalytic center is located adjacent tot or within the membrane | Bacillus subtilis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-sigmaK + H2O | Bacillus subtilis | processing of prosigmaK, which is a protein involved in sporulation | sigmaK + 20 amino acid peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
wild-type strain PY79 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
self-cleaves Val52-/-Asn53, Ala62-/-Phe63 and Val74-/-Thr75 at the N-terminus of SpoIVB | D137 is essential for catalytic activity, mechanism model, enzyme contains conserved metalloprotease sequence motifs HEXXH and NPDG, which are expected to form the catalytic center and are required for catalytic activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-sigmaK + H2O | processing of prosigmaK, which is a protein involved in sporulation | Bacillus subtilis | sigmaK + 20 amino acid peptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M50.002 | Merops-ID | Bacillus subtilis |
SpolVFB | - |
Bacillus subtilis |
stage IV sporulation protein FB | - |
Bacillus subtilis |