Any feedback?
Literature summary for 3.4.21.113 extracted from
- Detection and in-cell selectivity profiling of the full-length West Nile virus NS2B/NS3 serine protease using membrane-anchored fluorescent substrates (2010), Biol. Chem., 391, 549-559.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S135A | inactive | West Nile virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DPNRKRGWPA + H2O | West Nile virus | - |
DPNRKR + GWPA | - |
? |  |
| DSSTKRGGSW + H2O | West Nile virus | preferred substrate, 33.5% cleavage, the active protease is able to cleave the substrate at the endoplasmic reticulum membrane in Huh-7 cells | DSSTKR + GGSW | - |
? | |
| FASGKRSQIG + H2O | West Nile virus | - |
FASGKR + SQIG | - |
? | |
| KPGLKRGGAK + H2O | West Nile virus | preferred substrate, 34.5% cleavage | KPGLKR + GGAK | - |
? | |
| LQYTKRGGVL + H2O | West Nile virus | - |
LQYTKR + GGVL | - |
? | |
| additional information | West Nile virus | the NS3 protease of West nile virus has a cleavage sequence preference of (K/R)R-GG | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| West Nile virus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DPNRKRGWPA + H2O | - |
West Nile virus | DPNRKR + GWPA | - |
? | |
| DSSTKRGGSW + H2O | preferred substrate, 33.5% cleavage, the active protease is able to cleave the substrate at the endoplasmic reticulum membrane in Huh-7 cells | West Nile virus | DSSTKR + GGSW | - |
? | |
| FASGKRSQIG + H2O | - |
West Nile virus | FASGKR + SQIG | - |
? | |
| KPGLKRGGAK + H2O | preferred substrate, 34.5% cleavage | West Nile virus | KPGLKR + GGAK | - |
? | |
| LQYTKRGGVL + H2O | - |
West Nile virus | LQYTKR + GGVL | - |
? | |
| additional information | the NS3 protease of West nile virus has a cleavage sequence preference of (K/R)R-GG | West Nile virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavivirus NS3 protease | - |
West Nile virus |
| NS2B/NS3 protease | - |
West Nile virus |
| NS3prohelx | NS3 is a bifunctional enzyme consisting of protease (NS3pro) and helicase (NS3hel) | West Nile virus |
| WNV NS2B/NS3 | - |
West Nile virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
