Literature summary for 3.4.21.113 extracted from

Chernov, A.V.; Shiryaev, S.A.; Aleshin, A.E.; Ratnikov, B.I.; Smith, J.W.; Liddington, R.C.; Strongin, A.Y.
The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase (2008), J. Biol. Chem., 283, 17270-17278.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	West Nile virus

Protein Variants

Protein Variants	Comment	Organism
H51A	mutant shows inactivated proteolytic activity	West Nile virus
K48A	to prevent its self-cleavage an autolytic site-deficient mutant is generated	West Nile virus
additional information	it is shown that both NS3 helicase and NS3 proteinase-helicase constructs are capable of unwinding both the DNA and the RNA templates. In contrast, the full-length NS2B-NS3 proteinase-helicase unwinds only the RNA templates, whereas its DNA unwinding activity is severely repressed	West Nile virus
additional information	using a construct consisting of the upstram NS2B sequence and the proteinase domain bearing a K48A mutation followed by the helicase domain and another constrcut lacking the helicase domain it is shown that the helicase domain does not significantly affect the proteolytic activity	West Nile virus

Organism

Organism	UniProt	Comment	Textmining
West Nile virus	Q3HM13	-	-

Purification (Commentary)

Purification (Comment)	Organism
using HiTrap Co2+-chelating chromatography	West Nile virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pGlu-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O	-	West Nile virus	?	-	?

Synonyms

Synonyms	Comment	Organism
NS3 proteinase	-	West Nile virus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	West Nile virus

Cofactor

Cofactor	Comment	Organism	Structure
additional information	the presence of the upstream virus-encoded NS2B cofactor is required for NS3 proteinase to exhibit its functional activity	West Nile virus

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Release 2023.1 (January 2023)