Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | cellular stress induced by tunicamycin does not regulate the production of St-2 | Homo sapiens | |
N-acetyl-leucinal-leucinal-norleucinal | increases the level of St-1 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
full length SKI-1 and its H249A mutant expressed in HEK-293 cells, M19 cells, HuH7 cells and Hep-G2 cells. Wild-type SKI-1 and mutants H249A, L925A and K948A expressed in SRD-12B and CHO-K1 cells. Wild-type SKI-1 or a secretable form of St-1 containing a signal peptide followed by a FLAG tag at the N terminus and a V5 tag at the C terminus overexpressed in CHO-K1 cells. rSt-1 coexpressed with protease inhibitors in CHO-K1 and Neuro-2a cells. Mutants I985A, I989L, M990L, M990A, M990I, Y994A, N995A, and double mutant Y994A/N995A expressed in CHO-K1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H249A | active site mutant, autocatalytic generation of St-1 is abrogated, St-2 fragment is generated at a level of ca. 15% | Homo sapiens |
I985A | has no effect on generation of St-2 | Homo sapiens |
I989L | results in more than 90% reduction in the generation of St-2 | Homo sapiens |
K948A | shedding site mutant | Homo sapiens |
L952A | shedding site mutant | Homo sapiens |
M990A | fails to generate St-2 | Homo sapiens |
M990I | fails to generate St-2 | Homo sapiens |
M990L | fails to generate St-2 | Homo sapiens |
additional information | in the triple shedding site mutant KLL/A autocatalytic generation of St-1 is abrogated, St-2 fragment is generated at a level of ca. 15% | Homo sapiens |
N995A | has no effect on generation of St-2 | Homo sapiens |
Y994A | has no effect on generation of St-2 | Homo sapiens |
Y994A/N995A | has no effect on generation of St-2 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride | inhibits SKI-1 and the autocatalytic generation of St-1 | Homo sapiens | |
brefeldin A | abrogates St-2 production | Homo sapiens | |
additional information | (Z-LL)2-chloromethylketone and PN1 have no effect on St-2 production. Overexpressed rSt-1 or rSt-2 impede ATF6 processing by SKI-1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | St-2 production | Homo sapiens | 5783 | - |
Golgi apparatus | most of membrane-bound SKI-1 and its stumps St-1 and St-2 localize to the Golgi and can enter the endosomal/lysosomal compartments but do not sort to the cell surface | Homo sapiens | 5794 | - |
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8500 | - |
St-2 protein, time-of-flight mass spectrometry | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
Huh-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATF6 precursor + H2O | rSt-1 and rSt-2 seem to affect the processing of ATF6 by SKI-1 | Homo sapiens | nuclear ATF6 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S1P | - |
Homo sapiens |
SKI-1 | - |
Homo sapiens |
SKI-1/S1P | - |
Homo sapiens |
subtilisin kexin isozyme-1 | - |
Homo sapiens |