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Literature summary for 3.4.21.109 extracted from

  • Wysocka, M.; Gruba, N.; Miecznikowska, A.; Popow-Stellmaszyk, J.; Guetschow, M.; Stirnberg, M.; Furtmann, N.; Bajorath, J.; Lesner, A.; Rolka, K.
    Substrate specificity of human matriptase-2 (2014), Biochimie, 97, 121-127.
    View publication on PubMed
Search for more literature for 3.4.21.109

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of c-Myc-tagged enzyme in HEK cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
hepatocyte growth factor activator inhibitor type 2 HAI-2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.35
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Gly-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens
24.5
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ser-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens
35.5
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ala-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane with a C-terminal protease domain directed to the extracellular space and a stem region with domains of different types, between the transmembrane domain near the N-terminus and the protease domain Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hemojuvelin + H2O Homo sapiens bone morphogenetic protein co-receptor ?
-
 ?
matriptase-2 + H2O Homo sapiens autocatalysis ?
-
 ?
additional information Homo sapiens the optimized peptide substrate sequence is Ile-Arg-Ala-Arg-Ser-Ala-Gly ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IU80
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant c-Myc-tagged enzyme from HEK cells by immunoaffinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ala-Tyr(3-NO2)-NH2 + H2O
-
 Homo sapiens ABZ-Ile-Arg-Ala-Arg + Ser-Ala-Ala-Tyr(3-NO2)-NH2
-
 ?
ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Gly-Tyr(3-NO2)-NH2 + H2O
-
 Homo sapiens ABZ-Ile-Arg-Ala-Arg + Ser-Ala-Gly-Tyr(3-NO2)-NH2
-
 ?
ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ser-Tyr(3-NO2)-NH2 + H2O
-
 Homo sapiens ABZ-Ile-Arg-Ala-Arg + Ser-Ala-Ser-Tyr(3-NO2)-NH2
-
 ?
hemojuvelin + H2O
-
 Homo sapiens ?
-
 ?
hemojuvelin + H2O bone morphogenetic protein co-receptor Homo sapiens ?
-
 ?
matriptase-2 + H2O autocatalysis Homo sapiens ?
-
 ?
additional information the optimized peptide substrate sequence is Ile-Arg-Ala-Arg-Ser-Ala-Gly Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
matriptase-2
-
 Homo sapiens
MT-2
-
 Homo sapiens
TMPRSS6
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.24
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ala-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens
2.66
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ser-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens
3.79
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Gly-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens

General Information

General Information Comment Organism
evolution matriptase-2 belongs to the family of type II transmembrane serine proteases Homo sapiens
additional information putative substrate binding mode, molecular docking, overview Homo sapiens
physiological function matriptase-2 is a regulatory protease controlling iron homeostasis Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ala-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens
109.8
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Ser-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens
450.4
-
 ABZ-Ile-Arg-Ala-Arg-Ser-Ala-Gly-Tyr(3-NO2)-NH2 pH and temperature not specified in the publication Homo sapiens