Cloned (Comment) | Organism |
---|---|
overexpression in stomach cancer cell line AZ521, Mat-AZ521 cells release an active form of matriptase into culture medium | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | when implanting enzyme overexpressing Mat-AZ521 cells into nude mice subcutaneously or intraperitoneally, Mat-AZ521 cells grew faster and produced much larger solid tumors than the control cells, overexpression shortened the survival time of tumor-bearing mice, number and the size of blood vessels in tumor tissues are significantly higher in the Mat-AZ521 tumors than in the control cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | complete inhibition at 5 mM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | matriptase-2 is a type II transmembrane serine protease | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, native enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | matriptase promotes tumor growth and angiogenesis by enhancing extracellular matrix degradation in tumor cell microenvironments, overview | ? | - |
? | |
stromelysin + H2O | Homo sapiens | activation | activated stromelysin + propeptide of stromelysin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from colon carcinoma cell line DLD-1 by gel filtration, dialysis, adsorption chromatography, abion exchange chromatography, and freeze-drying | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | - |
Homo sapiens | - |
colon carcinoma cell | - |
Homo sapiens | - |
DLD-1 cell | - |
Homo sapiens | - |
epithelial cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | matriptase promotes tumor growth and angiogenesis by enhancing extracellular matrix degradation in tumor cell microenvironments, overview | Homo sapiens | ? | - |
? | |
stromelysin + H2O | activation | Homo sapiens | activated stromelysin + propeptide of stromelysin | - |
? | |
stromelysin + H2O | i.e. MMP-3 or matrix metalloproteinase-3, recombinant human substrate, activation | Homo sapiens | activated stromelysin + propeptide of stromelysin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, native enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |