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Literature summary for 3.4.21.109 extracted from

  • Jin, X.; Yagi, M.; Akiyama, N.; Hirosaki, T.; Higashi, S.; Lin, C.Y.; Dickson, R.B.; Kitamura, H.; Miyazaki, K.
    Matriptase activates stromelysin (MMP-3) and promotes tumor growth and angiogenesis (2006), Cancer Sci., 97, 1327-1334.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in stomach cancer cell line AZ521, Mat-AZ521 cells release an active form of matriptase into culture medium Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information when implanting enzyme overexpressing Mat-AZ521 cells into nude mice subcutaneously or intraperitoneally, Mat-AZ521 cells grew faster and produced much larger solid tumors than the control cells, overexpression shortened the survival time of tumor-bearing mice, number and the size of blood vessels in tumor tissues are significantly higher in the Mat-AZ521 tumors than in the control cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate complete inhibition at 5 mM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane matriptase-2 is a type II transmembrane serine protease Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
x * 75000, native enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens matriptase promotes tumor growth and angiogenesis by enhancing extracellular matrix degradation in tumor cell microenvironments, overview ?
-
?
stromelysin + H2O Homo sapiens activation activated stromelysin + propeptide of stromelysin
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from colon carcinoma cell line DLD-1 by gel filtration, dialysis, adsorption chromatography, abion exchange chromatography, and freeze-drying Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell
-
Homo sapiens
-
colon carcinoma cell
-
Homo sapiens
-
DLD-1 cell
-
Homo sapiens
-
epithelial cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information matriptase promotes tumor growth and angiogenesis by enhancing extracellular matrix degradation in tumor cell microenvironments, overview Homo sapiens ?
-
?
stromelysin + H2O activation Homo sapiens activated stromelysin + propeptide of stromelysin
-
?
stromelysin + H2O i.e. MMP-3 or matrix metalloproteinase-3, recombinant human substrate, activation Homo sapiens activated stromelysin + propeptide of stromelysin
-
?

Subunits

Subunits Comment Organism
? x * 75000, native enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens