Protein Variants | Comment | Organism |
---|---|---|
additional information | small interfering RNAs for matriptase efficiently block both the matriptase expression and the cleavage of IGBP-rP1 in OVISE cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride | inhibition of IGFBP-rP1 processing | Homo sapiens | |
Aprotinin | inhibition of IGFBP-rP1 processing | Homo sapiens | |
hepatocyte growth factor activator inhibitor-1 | HAI-1, forms complexes wih the enzyme in vivo | Homo sapiens | |
additional information | no inhibition of IGFBP-rP1 processing by leupeptin, pepstatin, and N-(R)-(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-l-3-(2'-naphthyl)alaninyl-l-alanine 2-aminoethyl amide, i.e. TAPI-1; not inhibited by leupeptin, pepstatin and N-(R)-(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-L-3-(2'-naphthyl)alaninyl-L-alanine 2-aminoethyl amide | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
membrane | - |
Homo sapiens | 16020 | - |
membrane | bound | Homo sapiens | 16020 | - |
soluble | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gelatin zymography, soluble active enzyme | Homo sapiens |
80000 | - |
immunoblotting, membrane-bound enzyme | Homo sapiens |
95000 | - |
gelatin zymography or immunoblotting, matripase/hepatocyte growth factor activator inhibitor complex | Homo sapiens |
110000 | - |
gelatin zymography or immunoblotting, matripase/hepatocyte growth factor activator inhibitor complex | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
insulin-like growth factor binding protein-related protein-1 + H2O | Homo sapiens | proteolytical cleavage on the cell surface of a a single-chain IGFB-rP1 to a two-chain form, consisting of a 25 kDa chain and an 8 kDa chain, changing its biological in modulation of cellular adhesion and growth in an IGF/insulin-dependent or independent manner, as well as tumor-suppressive activity, the soluble form of active matriptase cleaves IGFBP-rP1 but HAI-1 or some other inhibitors block its activity in culture medium, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by molecular-sieve chromatography, reverse-phase HPLC | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
DLD-1 cell | - |
Homo sapiens | - |
DLD-1 cell | major amount of matripase | Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
HSC-4 cell | - |
Homo sapiens | - |
MKN-45 cell | major amount of matripase | Homo sapiens | - |
MKN-45 cell | gastric carcinoma cells, high level expression of IGFBP-rP1-cleaving enzyme | Homo sapiens | - |
additional information | no IGFBP-rP1 processing activity in HSC-4 cells, HEK-293 cells, HLE cells, MKN-45 cells, HT-1080 cells, EJ-1 cells, and ECV-304 cells, the soluble form of active matriptase cleaves IGFBP-rP1 but HAI-1 or some other inhibitors block its activity in culture medium | Homo sapiens | - |
ovary adenocarcinoma cell line | major amount of matripase | Homo sapiens | - |
OVISE cell | ovarian clear cell adenocarcinoma cells, high level expression of IGFBP-rP1-cleaving enzyme | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
insulin growth factor binding protein-related protein-1 + H2O | cleaved by the soluble form of active matripase | Homo sapiens | ? | - |
? | |
insulin-like growth factor binding protein-related protein-1 + H2O | proteolytical cleavage on the cell surface of a a single-chain IGFB-rP1 to a two-chain form, consisting of a 25 kDa chain and an 8 kDa chain, changing its biological in modulation of cellular adhesion and growth in an IGF/insulin-dependent or independent manner, as well as tumor-suppressive activity, the soluble form of active matriptase cleaves IGFBP-rP1 but HAI-1 or some other inhibitors block its activity in culture medium, overview | Homo sapiens | ? | - |
? | |
insulin-like growth factor binding protein-related protein-1 + H2O | i.e. IGFBP-rP1 or angiomodulin or mac25 | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000-80000, single-chain latent enzyme, SDS-PAGE, x * 95000-110000, active enzyme in complex with inhibitor HAI-1, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
membrane-type serine proteinase matripase | - |
Homo sapiens |
membrane-type serine proteinase matriptase | - |
Homo sapiens |
MT-SP1 | - |
Homo sapiens |