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Literature summary for 3.4.21.108 extracted from
- Role of conserved regulatory loop residues in allosteric propagation of serine protease HtrA2 (2022), Biochem. Biophys. Res. Commun., 594, 63-68.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| BIR2 | - |
Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F131A | the mutant shows strongly decreased catalytyic efficiency in the presence of activator peptide BIR2 compared to the wild type enzyme | Homo sapiens |
| P148A | the mutant shows strongly increased catalytyic efficiency in the presence of activator peptide BIR2 compared to the wild type enzyme | Homo sapiens |
| R147A | inactive | Homo sapiens |
| S173A | inactive | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000974 | - |
beta-casein | wild type enzyme, in the absence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00136 | - |
beta-casein | mutant enzyme P148A, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00175 | - |
beta-casein | mutant enzyme P148A, in the absence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00231 | - |
beta-casein | wild type enzyme, in the absence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00333 | - |
beta-casein | mutant enzyme F131A, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O43464 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-casein + H2O | - |
Homo sapiens | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| high temperature requirement protease A2 | - |
Homo sapiens |
| HtrA2 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00067 | - |
beta-casein | mutant enzyme F131A, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0011 | - |
beta-casein | wild type enzyme, in the absence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0018 | - |
beta-casein | mutant enzyme P148A, in the absence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.003 | - |
beta-casein | wild type enzyme, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0051 | - |
beta-casein | mutant enzyme P148A, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
beta-casein | mutant enzyme F131A, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 0.27 | - |
beta-casein | wild type enzyme, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 1.03 | - |
beta-casein | mutant enzyme P148A, in the absence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 1.12 | - |
beta-casein | wild type enzyme, in the absence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.73 | - |
beta-casein | mutant enzyme P148A, in the presence of activator peptide BIR2, pH and temperature not specified in the publication | Homo sapiens |
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