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Literature summary for 3.4.21.108 extracted from

  • Zurawa-Janicka, D.; Jarzab, M.; Polit, A.; Skorko-Glonek, J.; Lesner, A.; Gitlin, A.; Gieldon, A.; Ciarkowski, J.; Glaza, P.; Lubomska, A.; Lipinska, B.
    Temperature-induced changes of HtrA2(Omi) protease activity and structure (2013), Cell Stress Chaperones, 18, 35-51.
    View publication on PubMed
Search for more literature for 3.4.21.108

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
F303W mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
F331W mutation in proease domain, mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
F331Y inactive Homo sapiens
I329N inactive Homo sapiens
L367W mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
L377W mutation in PDZ domain, mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
R432L mutation in PDZ domain, significant increase in activity Homo sapiens
V226K mutation in protease domain, significant increase in activity Homo sapiens
V226K E429L salt link formation is no longer possible, the increase of activity observed for mutant V226K ceased to exist Homo sapiens
V226W mutation in proease domain, proteolytic activity similar to wild-type Homo sapiens
V325D inactive Homo sapiens
V364W mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
Y361W mutation in proease domain, proteolytic activity similar to wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00158
-
 (7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide pH 8.0, 45°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43464
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
HtrA2(Omi)
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 activity increases about 14fold between 25 and 45 °C Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 activity is practically negligible at 20 °C Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 upon an increase in temperature the HtrA2 structure relaxes, the PDZ-protease interface becomes more exposed to the solvent, and significant conformational changes involving both domains occur at and above 30 °C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 (7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide pH 8.0, 45°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
31.13
-
 (7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide pH 8.0, 45°C Homo sapiens