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Literature summary for 3.4.21.108 extracted from

  • Kuninaka, S.; Iida, S.I.; Hara, T.; Nomura, M.; Naoe, H.; Morisaki, T.; Nitta, M.; Arima, Y.; Mimori, T.; Yonehara, S.; Saya, H.
    Serine protease Omi/HtrA2 targets WARTS kinase to control cell proliferation (2007), Oncogene, 26, 2395-2406.
    View publication on PubMed
Search for more literature for 3.4.21.108

Activating Compound

Activating Compound Comment Organism Structure
additional information apoptotic stimuli induce the activation of Omi and the consequent digestion of WTS by a caspase-independent mechanism Mus musculus
additional information activation of Omi by UV radiation Homo sapiens
staurosporine activates protease activity of Omi leading to cell death. Induces cell death in wild-type MEFs but not in caspase-9-deficient MEFs Mus musculus
staurosporine
-
 Homo sapiens
WTS activator of Omi protease Mus musculus

Application

Application Comment Organism
additional information a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death Mus musculus
additional information a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death Homo sapiens
additional information a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death synthetic construct

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli (BL21) transformed with pRSET containing cDNA for full-length wild-type Omi or mutant synthetic construct

Protein Variants

Protein Variants Comment Organism
S306A inactive, has no effect on the abundance of WTS synthetic construct
Y361A mature Omi with PDZ domain mutation, cannot interact with C-terminus of WTS, fails to cleave full-length WTS synthetic construct

Inhibitors

Inhibitors Comment Organism Structure
N-tosyllysine chloromethyl ketone
-
 Homo sapiens
N-tosyllysine chloromethyl ketone blocks ability of wild-type Omi to proteolyse WTS synthetic construct
siRNA reduces endogenous Omi expression in HeLa cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-
nucleus
-
 Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
synthetic construct
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immunopurified from HEK-293 T cells Homo sapiens
on Ni2+ agarose beads synthetic construct

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-
HeLa cell full length Omi present in mitochondria and nucleus Homo sapiens
-
L-929 cell
-
 Mus musculus
-
MEF cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
WTS + H2O is a non-apoptotic substrate of Omi Mus musculus ?
-
 ?
WTS + H2O
-
 Homo sapiens ?
-
 ?
WTS + H2O
-
 synthetic construct ?
-
 ?

Synonyms

Synonyms Comment Organism
HtrA2
-
 Mus musculus
HtrA2
-
 Homo sapiens
HtrA2
-
 synthetic construct
Omi
-
 Mus musculus
Omi
-
 Homo sapiens
Omi
-
 synthetic construct
Omi protease
-
 Mus musculus
Omi protease
-
 Homo sapiens
Omi protease
-
 synthetic construct
Omi/HtrA2
-
 Mus musculus
Omi/HtrA2
-
 Homo sapiens
Omi/HtrA2
-
 synthetic construct
serine protease Omi/HtrA2
-
 Mus musculus
serine protease Omi/HtrA2
-
 Homo sapiens
serine protease Omi/HtrA2
-
 synthetic construct