Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.108 extracted from

  • Plun-Favreau, H.; Klupsch, K.; Moisoi, N.; Gandhi, S.; Kjaer, S.; Frith, D.; Harvey, K.; Deas, E.; Harvey, R.J.; McDonald, N.; Wood, N.W.; Martins, L.M.; Downward, J.
    The mitochondrial protease HtrA2 is regulated by Parkinsons disease-associated kinase PINK1 (2007), Nat. Cell Biol., 9, 1243-1252.
    View publication on PubMed
Search for more literature for 3.4.21.108

Activating Compound

Activating Compound Comment Organism Structure
additional information phosphorylation of HtrA2 increases its proteolytic activtiy. PINK1 modulates the levels of phosphorylated HtrA2. Activation of MEKK3 induces efficient phosphorylation of both full-length and processed HtrA2 on Ser142. In vitro, p38 is also able to phosphorylate around Ser142 Homo sapiens

Application

Application Comment Organism
medicine HtrA2 is regulated by Parkinson's disease-associated kinase PINK1. HtrA2 interacts with PINK1 and that both are components of the same stress-sensing pathway Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
TAP-tagged HtrA2 stably expressed in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
A141S mutation in HtrA2 identified in patients with Parkinson's disease Homo sapiens
G399S mutation in HtrA2 identified in patients with Parkinson's disease, ability of p38gamma to phosphorylate the peptide is decreased Homo sapiens
additional information HtrA2 knockout MEFs, no endogenous PINK1. Show decreased mitochondrial membrane potential Mus musculus
S142A considerably less phosphorylated by p38gamma in vitro. Markedly lower protease activity than its acidic counterpart Homo sapiens
S142D phospho-mimetic HtrA2-mutant, increases the basal ability of HtrA2 to cleave a fluorogenic substrate peptide by about 2fold Homo sapiens
S400A considerably less phosphorylated by p38gamma in vitro. Markedly lower protease activity than its acidic counterpart Homo sapiens
S400D phospho-mimetic HtrA2-mutant, increases the basal ability of HtrA2 to cleave a fluorogenic substrate peptide by about 3fold Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information HtrA2 phosphorylation is decreased in brains of patients with Parkinson's disease carrying mutations in PINK1 (Y431H and C575R) Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion
-
 Mus musculus 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
MEF cell
-
 Mus musculus
-
SH-SY5Y cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
Omi
-
 Homo sapiens
Omi
-
 Mus musculus
serine protease HtrA2
-
 Homo sapiens
serine protease HtrA2
-
 Mus musculus