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Literature summary for 3.4.21.108 extracted from

  • Kim, G.Y.; Nam, M.K.; Kim, S.S.; Kim, H.Y.; Lee, S.K.; Rhim, H.
    A simple and rapid strategy for the molecular cloning and monitoring of mouse HtrA2 serine protease (2008), Biotechnol. Lett., 30, 397-403.
    View publication on PubMed
Search for more literature for 3.4.21.108

Application

Application Comment Organism
additional information simple and rapid strategy for molecular cloning using a gel-free and antibiotic selection method, which allows for the complete elimination of DNA extraction by gel electrophoresis, and thus has several advantages over gel-based cloning methods, including a cloning efficiency that is approximately 10times higher due to the prevention of ethidium bromide ultraviolet-induced DNA damage and contamination with ligase inhibitors, the amount of plasmid DNA required is approximately five times less, and cloning time is several hours less Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
into plasmid pEGFPN3 and plasmid pGEX-4T1-FLAG. Expression of GST-HtrA2 fusion protein in BL21 cells and in HEK-293 cells Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 by immunoblotting Mus musculus
61790
-
 GST-HtrA2, calculated by the Compute pI/Mw method at the ExPASy proteomics server of the Swiss Institute of Bioinformatics Mus musculus
63000
-
 GST-HtrA2, by immunoblotting Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity purified Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O cleaved to approximately 50% by GST-HtrA2 after a cleavage reaction for 1 h Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
HtrA2 serine protease
-
 Mus musculus