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Literature summary for 3.4.21.108 extracted from

  • Seong, Y.M.; Park, H.J.; Seong, G.H.; Choi, J.Y.; Yoon, S.J.; Min, B.R.; Kang, S.; Rhim, H.
    N-terminal truncation circumvents proteolytic degradation of the human HtrA2/Omi serine protease in Escherichia coli: rapid purification of a proteolytically active HtrA2/Omi (2004), Protein Expr. Purif., 33, 200-208.
    View publication on PubMed
Search for more literature for 3.4.21.108

Cloned(Commentary)

Cloned (Comment) Organism
barely expressed full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21 Homo sapiens
successful expression of full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21 as compared to human HtrA2/Omi Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000 44000 low molecular masses detected due to full-length HtrA2/Omi regions or amino acid residues that are very susceptible to a high degree of proteolytic events in Escherichia coli expression systems Homo sapiens
77000
-
-
 Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens O43464
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
rapid purification of full-length HtrA2/Omi as a GST fusion under non-denaturing conditions by selective binding to glutathione-Sepharose 4B beads Homo sapiens
rapid purification of GST-HtrA2/Omi fusion proteins under non-denaturing conditions by selective binding to glutathione-Sepharose 4B beads Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain from fetus Homo sapiens
-

Storage Stability

Storage Stability Organism
-70°C, Tris-HCl buffer, pH 7.6, 20% glycerol, several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
HtrA2/Omi
-
 Mus musculus
HtrA2/Omi serine protease
-
 Homo sapiens