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Literature summary for 3.4.21.104 extracted from
- Functional characterization of complement proteases C1s/mannan-binding lectin-associated serine protease-2 (MASP-2) chimeras reveals the higher C4 recognition efficacy of the MASP-2 complement control protein modules (2005), J. Biol. Chem., 280, 41811-41818.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C1s(MASP-2CCP1/2) | hybrid C1s/MASP-2 molecule, swapped complement control protein, 21-27fold higher kcat/Km-ratio for complement C4 than C1s(MASP-2SP) | Homo sapiens |
| C1s(MASP-2SP) | hybrid C1s/MASP-2 molecule, swapped serine protease, 21-27fold lower kcat/Km-ratio for complement C4 than C1s(MASP-2CCP1/2) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000085 | - |
Complement component C4 | wild-type, 37°C | Homo sapiens | |
| 0.000147 | - |
Complement component C4 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens | |
| 0.0028 | - |
Complement component C4 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
| 0.0052 | - |
Complement component C2 | wild-type, 37°C | Homo sapiens | |
| 0.0073 | - |
Complement component C2 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
| 0.0092 | - |
Complement component C2 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
29000 + 60000, chimera C1s(MASP-2SP), SDS-PAGE | Homo sapiens |
| 78000 | - |
x * 78000, chimera C1s(MASP-2CCP1/2), SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O00187 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chimeric proteins C1s(MASP-2CCP1/2) and C1s(MASP-2SP) | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C2 + H2O | 37°C | Homo sapiens | ? | the complement C3 convertase C4bC2b is generated by cleavage of complement C4 and complement C2 | ? |  |
| complement component C4 + H2O | 37°C | Homo sapiens | ? | the complement C3 convertase C4bC2b is generated by cleavage of complement C4 and complement C2 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, chimera C1s(MASP-2CCP1/2), SDS-PAGE | Homo sapiens |
| dimer | 29000 + 60000, chimera C1s(MASP-2SP), SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mannan-binding lectin-associated serine protease-2 | - |
Homo sapiens |
| MASP-2 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
Complement component C4 | wild-type, 37°C | Homo sapiens | |
| 2.9 | - |
Complement component C4 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
| 3.2 | - |
Complement component C4 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens | |
| 5.1 | - |
Complement component C2 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens | |
| 5.2 | - |
Complement component C2 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
| 5.6 | - |
Complement component C2 | wild-type, 37°C | Homo sapiens |
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