Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.104 extracted from

  • Ambrus, G.; Gal, P.; Kojima, M.; Szilagyi, K.; Balczer, J.; Antal, J.; Graf, L.; Laich, A.; Moffatt, B.E.; Schwaeble, W.; Sim, R.B.; Zavodszky, P.
    Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments (2003), J. Immunol., 170, 1374-1382.
    View publication on PubMed
Search for more literature for 3.4.21.104

Cloned(Commentary)

Cloned (Comment) Organism
MASP-2 and truncated fragments of the catalytic region Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha-2-Macroglobulin forms a stable complex with the enzyme, inhibits at a large molar excess, 40fold Homo sapiens
C1-inhibitor forms a stable complex with the enzyme, 1-3fold excess completely inactivates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 Complement component C4 37°C, pH 7.4 Homo sapiens
4
-
 Complement component C2 37°C, pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens complement C3 is not a natural substrate ?
-
 ?
Protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C2 + H2O SP domain alone can autoactivate and cleave C2 Homo sapiens ?
-
 ?
complement component C3i + H2O
-
 Homo sapiens ?
-
 ?
complement component C4 + H2O SP domain is able to cleave C4, but the presence of the CCP2 domain significantly increases the efficiency Homo sapiens ?
-
 ?
additional information hardly any detectable activity on complement C3 Homo sapiens ?
-
 ?
additional information complement C3 is not a natural substrate Homo sapiens ?
-
 ?
Protein + H2O
-
 Homo sapiens ?
-
 ?
Protein + H2O cleaves oligopeptides that contain either Arg or Lys at their P1 positions Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MASP-2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 Complement component C4 37°C, pH 7.4 Homo sapiens
1.9
-
 Complement component C2 37°C, pH 7.4 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000088
-
 C1-inhibitor 37°C, pH 7.4 Homo sapiens