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Literature summary for 3.4.21.103 extracted from
- The Physarum polycephalum php gene encodes a unique cold-adapted serine-carboxyl peptidase, physarolisin II (2003), FEBS Lett., 546, 340-344.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Physarum polycephalum |
General Stability
| General Stability | Organism |
|---|---|
| the active enzyme is markedly unstable due to rapid autolysis, t1/2: 5 min at 18°C, pH 4.2 | Physarum polycephalum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | 23% inhibition by 0.5 mM, 76% inhibition by 10 mM | Physarum polycephalum |  |
| additional information | no inhibition by 0.01 mM pepstatin, 10 mM Ala-Ala-Phe-CH2Cl, 0.2 mM diazoacetyl-DL-norleucine methyl ester, 10 mM EDTA or 10 mM EGTA | Physarum polycephalum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physarum polycephalum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glucagon + H2O | - |
Physarum polycephalum | ? | - |
? | |
| KPIEFF(NO2)RL + H2O | - |
Physarum polycephalum | ? | - |
? | |
| additional information | no hydrolysis of hemoglobin, casein, azocoll and Ala-Ala-Phe-7-amido-4-methylcoumarin | Physarum polycephalum | ? | - |
? | |
| oxidized insulin B + H2O | - |
Physarum polycephalum | ? | - |
? | |
| Substance P + H2O | - |
Physarum polycephalum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| physarolysin II | - |
Physarum polycephalum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 16 | 22 | - |
Physarum polycephalum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 3 | 30 | 3°C: about 50% of maximal activity, 30°C: about 60% of maximal activity, hydrolysis of oxidized insulin B chain | Physarum polycephalum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | - |
pH 4.2, t1/2: 5 min | Physarum polycephalum |
| 37 | - |
pH 4.2, t1/2: 6 h | Physarum polycephalum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
hydrolysis of oxidized insulin B chain | Physarum polycephalum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.7 | 5.3 | pH 3.7: about 40% of maximal actuvity, pH 5.3: about 35% of maximal activity, hydrolysis of oxidized insulin B chain | Physarum polycephalum |
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Release 2023.1 (January 2023)
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