Literature summary for 3.4.21.102 extracted from

Keiler, K.C.; Silber, K.R.; Downard, K.M.; Papayannopoulos, I.A.; Biemann, K.; Sauer, R.T.
C-terminal specific protein degradation: activity and substrate specificity of the Tsp protease (1995), Protein Sci., 4, 1507-1515.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	Ac-WARAAARAAARBAAB	-	Escherichia coli
0.05	-	Arc repressor	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	TspH6, enzyme protein with six additional His residues at the C-terminus, calculation from sequence of cDNA	Escherichia coli
78500	-	TspH6, enzyme protein with six additional His residues at the C-terminus, sedimentation equilibrium	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Protein + H2O	Escherichia coli	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ac-WARAAARAAARBAAB + H2O	i.e. peptide BAS9	Escherichia coli	?	-	?
Ac-WARAAARAAARBGGB + H2O	i.e. peptide BAS10	Escherichia coli	?	-	?
Arc repressor + H2O	C-terminal sequence: GRIGA, endoproteolyric cleavage	Escherichia coli	?	-	?
Protein + H2O	-	Escherichia coli	?	-	?
variant of the lambda repressor + H2O	C-terminal sequence: WVAAA, endoproteolytic cleavage	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
monomer	sedimentation equilibrium sequence of cDNA	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.19	-	Arc repressor	-	Escherichia coli
3.7	-	Ac-WARAAARAAARBAAB	-	Escherichia coli

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Release 2023.1 (January 2023)