BRENDA - Enzyme Database show
show all sequences of 3.4.21.101

Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis

Oyama, H.; Abe, S.I.; Ushiyama, S.; Takahashi, S.; Oda, K.; J. Biol. Chem. 274, 27815-27822 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Xanthomonas sp.
Engineering
Amino acid exchange
Commentary
Organism
D348A
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
Xanthomonas sp.
DELTA192
pro-region mutant enzyme
Xanthomonas sp.
S169A
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
Xanthomonas sp.
S79A
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
Xanthomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme DELTA192
Xanthomonas sp.
0.0111
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E230A
Xanthomonas sp.
0.0188
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E235A
Xanthomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xanthomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant mutant enzymes S79A, S169A and D348A
Xanthomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
casein + H2O
-
651950
Xanthomonas sp.
?
-
651950
Xanthomonas sp.
?
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651950
Xanthomonas sp.
Lys-Pro-Ala-Leu-Phe + p-nitrophenylalanine-Arg-Leu
-
651950
Xanthomonas sp.
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E235A
Xanthomonas sp.
0.9
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme DELTA192
Xanthomonas sp.
21.8
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E230A
Xanthomonas sp.
34.9
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E235A
Xanthomonas sp.
68.4
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme DELTA192
Xanthomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Xanthomonas sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D348A
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
Xanthomonas sp.
DELTA192
pro-region mutant enzyme
Xanthomonas sp.
S169A
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
Xanthomonas sp.
S79A
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
Xanthomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme DELTA192
Xanthomonas sp.
0.0111
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E230A
Xanthomonas sp.
0.0188
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E235A
Xanthomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant mutant enzymes S79A, S169A and D348A
Xanthomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
casein + H2O
-
651950
Xanthomonas sp.
?
-
651950
Xanthomonas sp.
?
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
651950
Xanthomonas sp.
Lys-Pro-Ala-Leu-Phe + p-nitrophenylalanine-Arg-Leu
-
651950
Xanthomonas sp.
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E235A
Xanthomonas sp.
0.9
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme DELTA192
Xanthomonas sp.
21.8
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E230A
Xanthomonas sp.
34.9
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E235A
Xanthomonas sp.
68.4
-
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme DELTA192
Xanthomonas sp.
Other publictions for EC 3.4.21.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665088
Oda
-
Sedolisin-B ...
Xanthomonas sp., Xanthomonas sp. T-22
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1888-1889
2004
-
-
1
-
-
-
2
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
1
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
2
1
1
-
-
2
-
-
-
-
-
-
-
-
-
6
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
649090
Wlodawer
Structural and enzymatic prope ...
Xanthomonas sp.
Acta Biochim. Pol.
50
81-102
2003
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651761
Narutaki
Subsite preferences of pepstat ...
Xanthomonas sp., Xanthomonas sp. T-22
J. Biochem.
125
75-81
1999
-
-
-
-
-
-
1
45
-
-
-
-
-
2
-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
45
-
-
-
-
-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
651950
Oyama
Identification of catalytic re ...
Xanthomonas sp.
J. Biol. Chem.
274
27815-27822
1999
-
-
1
-
4
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
137236
Oda
Cloning, nucleotide sequence, ...
Pseudomonas sp., Pseudomonas sp. No. 101
J. Biol. Chem.
269
26518-26524
1994
-
-
1
-
-
-
-
4
-
-
1
-
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
137232
Oda
Purification and properties of ...
Pseudomonas sp., Pseudomonas sp. No. 101
Biochim. Biophys. Acta
923
463-469
1987
-
-
-
-
-
-
5
-
-
-
2
-
-
5
-
1
1
-
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
2
-
-
-
1
1
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
137235
Oda
-
Purification and properties of ...
Xanthomonas sp.
Agric. Biol. Chem.
51
3073-3080
1987
-
-
-
-
-
-
4
-
-
1
2
-
-
1
-
1
1
-
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
2
-
-
-
1
1
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-