BRENDA - Enzyme Database
show all sequences of 3.4.21.101

Purification and properties of an S-PI(pepstatin Ac)-insensitive carboxyl proteinase from a Xanthomonas sp. Bacterium

Oda, K.; Nakazima, T.; Terashita, T.; Suzuki, K.i.; Murao, S.; Agric. Biol. Chem. 51, 3073-3080 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Xanthomonas sp.
Microbial alkaline protease inhibitor alpha
weak
Xanthomonas sp.
additional information
diazoacetyl-DL-norleucine methyl ester; divalent cations; methyl 2-diazoacetamidohexanoate; not: pepstatin
Xanthomonas sp.
PCMB
slight
Xanthomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no inhibition or activation by divalent cations
Xanthomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration
Xanthomonas sp.
41000
-
1 * 41000, SDS-PAGE
Xanthomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Xanthomonas sp.
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
contains less than 0.05% carbohydrate
Xanthomonas sp.
Purification (Commentary)
Purification (Commentary)
Organism
-
Xanthomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Xanthomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Xanthomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Acid-denatured hemoglobin + H2O
-
137235
Xanthomonas sp.
?
-
-
-
?
casein + H2O
-
137235
Xanthomonas sp.
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 41000, SDS-PAGE
Xanthomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Xanthomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 3.5-5.3, 20 h, stable
Xanthomonas sp.
50
-
pH 4.8, 15 min, stable below
Xanthomonas sp.
60
-
pH 4.8, 15 min, complete loss of activity
Xanthomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7
-
casein, acid-denatured hemoglobin
Xanthomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
5.3
37°C, 20 h, stable
Xanthomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Xanthomonas sp.
Microbial alkaline protease inhibitor alpha
weak
Xanthomonas sp.
additional information
diazoacetyl-DL-norleucine methyl ester; divalent cations; methyl 2-diazoacetamidohexanoate; not: pepstatin
Xanthomonas sp.
PCMB
slight
Xanthomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no inhibition or activation by divalent cations
Xanthomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration
Xanthomonas sp.
41000
-
1 * 41000, SDS-PAGE
Xanthomonas sp.
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
contains less than 0.05% carbohydrate
Xanthomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Xanthomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Xanthomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Xanthomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Acid-denatured hemoglobin + H2O
-
137235
Xanthomonas sp.
?
-
-
-
?
casein + H2O
-
137235
Xanthomonas sp.
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 41000, SDS-PAGE
Xanthomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Xanthomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 3.5-5.3, 20 h, stable
Xanthomonas sp.
50
-
pH 4.8, 15 min, stable below
Xanthomonas sp.
60
-
pH 4.8, 15 min, complete loss of activity
Xanthomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7
-
casein, acid-denatured hemoglobin
Xanthomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
5.3
37°C, 20 h, stable
Xanthomonas sp.
Other publictions for EC 3.4.21.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665088
Oda
-
Sedolisin-B ...
Xanthomonas sp., Xanthomonas sp. T-22
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1888-1889
2004
-
-
1
-
-
-
2
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
6
1
3
-
-
-
1
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
2
1
1
-
-
2
-
-
-
-
-
-
-
-
-
6
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
649090
Wlodawer
Structural and enzymatic prope ...
Xanthomonas sp.
Acta Biochim. Pol.
50
81-102
2003
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651761
Narutaki
Subsite preferences of pepstat ...
Xanthomonas sp., Xanthomonas sp. T-22
J. Biochem.
125
75-81
1999
-
-
-
-
-
-
1
45
-
-
-
-
-
2
-
-
-
-
-
-
-
-
51
-
1
-
-
-
59
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
45
-
-
-
-
-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
651950
Oyama
Identification of catalytic re ...
Xanthomonas sp.
J. Biol. Chem.
274
27815-27822
1999
-
-
1
-
4
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
137236
Oda
Cloning, nucleotide sequence, ...
Pseudomonas sp., Pseudomonas sp. No. 101
J. Biol. Chem.
269
26518-26524
1994
-
-
1
-
-
-
-
4
-
-
1
-
-
5
-
-
-
-
-
-
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
137232
Oda
Purification and properties of ...
Pseudomonas sp., Pseudomonas sp. No. 101
Biochim. Biophys. Acta
923
463-469
1987
-
-
-
-
-
-
5
-
-
-
2
-
-
5
-
1
1
-
-
-
1
-
4
1
-
1
-
3
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
2
-
-
-
1
1
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
137235
Oda
-
Purification and properties of ...
Xanthomonas sp.
Agric. Biol. Chem.
51
3073-3080
1987
-
-
-
-
-
-
4
-
-
1
2
-
-
1
-
1
1
-
-
1
1
-
2
1
-
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
2
-
-
-
1
1
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-