BRENDA - Enzyme Database show
show all sequences of 3.4.21.101

Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium

Oda, K.; Sugitani, M.; Fukuhara, K.; Murao, S.; Biochim. Biophys. Acta 923, 463-469 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Pseudomonas sp.
chymostatin
weak
Pseudomonas sp.
HgCl2
slight
Pseudomonas sp.
additional information
diisopropyl fluorophosphate; EDTA; iodoacetic acid; methyl 2-diazoacetamidohexanoate; not: pepstatin; p-chloromercuribenzenesulfonic acid; phenylmethanesulfonyl fluoride
Pseudomonas sp.
PCMB
slight
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Pseudomonas sp.
43000
-
1 * 43000, SDS-PAGE
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. No. 101
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
contains less than 0.05% carbohydrate
Pseudomonas sp.
Purification (Commentary)
Commentary
Organism
strain No. 101
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Acid-denatured hemoglobin + H2O
-
137232
Pseudomonas sp.
?
-
-
-
?
Acid-denatured hemoglobin + H2O
-
137232
Pseudomonas sp. No. 101
?
-
-
-
?
casein + H2O
-
137232
Pseudomonas sp.
?
-
-
-
?
casein + H2O
-
137232
Pseudomonas sp. No. 101
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 4.0-5.5, 15 h, stable
Pseudomonas sp.
45
-
15 min, stable below
Pseudomonas sp.
65
-
15 min, complete loss of activity
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
acid-denatured hemoglobin, casein
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
5.5
37°C, 15 h, stable
Pseudomonas sp.
4.8
-
15 min, stable below 50°C, complete loss of activity at 60°C
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Pseudomonas sp.
chymostatin
weak
Pseudomonas sp.
HgCl2
slight
Pseudomonas sp.
additional information
diisopropyl fluorophosphate; EDTA; iodoacetic acid; methyl 2-diazoacetamidohexanoate; not: pepstatin; p-chloromercuribenzenesulfonic acid; phenylmethanesulfonyl fluoride
Pseudomonas sp.
PCMB
slight
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Pseudomonas sp.
43000
-
1 * 43000, SDS-PAGE
Pseudomonas sp.
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
contains less than 0.05% carbohydrate
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
strain No. 101
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Acid-denatured hemoglobin + H2O
-
137232
Pseudomonas sp.
?
-
-
-
?
Acid-denatured hemoglobin + H2O
-
137232
Pseudomonas sp. No. 101
?
-
-
-
?
casein + H2O
-
137232
Pseudomonas sp.
?
-
-
-
?
casein + H2O
-
137232
Pseudomonas sp. No. 101
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 4.0-5.5, 15 h, stable
Pseudomonas sp.
45
-
15 min, stable below
Pseudomonas sp.
65
-
15 min, complete loss of activity
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
acid-denatured hemoglobin, casein
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
5.5
37°C, 15 h, stable
Pseudomonas sp.
4.8
-
15 min, stable below 50°C, complete loss of activity at 60°C
Pseudomonas sp.
Other publictions for EC 3.4.21.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665088
Oda
-
Sedolisin-B ...
Xanthomonas sp., Xanthomonas sp. T-22
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1888-1889
2004
-
-
1
-
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-
2
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
1
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
2
1
1
-
-
2
-
-
-
-
-
-
-
-
-
6
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
649090
Wlodawer
Structural and enzymatic prope ...
Xanthomonas sp.
Acta Biochim. Pol.
50
81-102
2003
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
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-
-
-
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1
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-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651761
Narutaki
Subsite preferences of pepstat ...
Xanthomonas sp., Xanthomonas sp. T-22
J. Biochem.
125
75-81
1999
-
-
-
-
-
-
1
45
-
-
-
-
-
2
-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
45
-
-
-
-
-
-
-
-
-
-
-
-
51
-
-
-
-
59
-
-
-
-
-
-
-
-
-
-
651950
Oyama
Identification of catalytic re ...
Xanthomonas sp.
J. Biol. Chem.
274
27815-27822
1999
-
-
1
-
4
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
137236
Oda
Cloning, nucleotide sequence, ...
Pseudomonas sp., Pseudomonas sp. No. 101
J. Biol. Chem.
269
26518-26524
1994
-
-
1
-
-
-
-
4
-
-
1
-
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
137232
Oda
Purification and properties of ...
Pseudomonas sp., Pseudomonas sp. No. 101
Biochim. Biophys. Acta
923
463-469
1987
-
-
-
-
-
-
5
-
-
-
2
-
-
5
-
1
1
-
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
2
-
-
-
1
1
-
-
1
-
4
1
1
-
3
-
1
-
2
-
-
-
-
-
-
-
137235
Oda
-
Purification and properties of ...
Xanthomonas sp.
Agric. Biol. Chem.
51
3073-3080
1987
-
-
-
-
-
-
4
-
-
1
2
-
-
1
-
1
1
-
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
2
-
-
-
1
1
-
1
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-