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Literature summary for 3.4.21.1 extracted from

  • Bibo-Verdugo, B.; Rojo-Arreola, L.; Navarrete-del-Toro, M.A.; Garcia-Carreno, F.
    A chymotrypsin from the digestive tract of California Spiny Lobster, Panulirus interruptus purification and biochemical characterization (2015), Mar. Biotechnol., 17, 416-427 .
    View publication on PubMed
Search for more literature for 3.4.21.1

Inhibitors

Inhibitors Comment Organism Structure
acetone 10-50%, significantly reduces residual activity to about 80 % at all tested concentrations Panulirus interruptus
chymostatin 0.02 mM, complete loss of activity Panulirus interruptus
DMSO 10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45% Panulirus interruptus
additional information E-64 and pepstatin A have no effect on enzyme activity Panulirus interruptus
phenylmethylsulfonyl fluoride 1 mM, 95% loss of activity Panulirus interruptus
Propanol reduces the residual activity to about 80 % when tested at concentrations of 10 and 30%. Activity is not affected using 50% Panulirus interruptus
TLCK 1 mM, 10% loss of activity Panulirus interruptus
Toluene 10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45% Panulirus interruptus
TPCK 1 mM, 10% loss of activity Panulirus interruptus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Panulirus interruptus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20100
-
 SDS-PAGE, enzyme is not heat-treated prior to electrophoresis Panulirus interruptus
28000
-
 SDS-PAGE, enzyme is incubated at 100°C for 10 min with and without DDT prior to electrophoresis Panulirus interruptus

Organism

Organism UniProt Comment Textmining
Panulirus interruptus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Panulirus interruptus

Source Tissue

Source Tissue Comment Organism Textmining
gastric juice
-
 Panulirus interruptus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 substrate: succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide, pH 8.0, 25°C Panulirus interruptus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bovine serum albumin + H2O
-
 Panulirus interruptus ?
-
 ?
Collagen + H2O
-
 Panulirus interruptus ?
-
 ?
additional information no activity with benzoyl-Arg-p-nitroanilide Panulirus interruptus ?
-
 ?
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide + H2O
-
 Panulirus interruptus succinyl-L-Ala-Ala-Pro-L-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Panulirus interruptus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Panulirus interruptus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 65 35°C: about 40% of maximal activity, 45°C: about 85% of maximal activity, 65°C: about 25% of maximal activity Panulirus interruptus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 60 min, about 20% loss of activity Panulirus interruptus
35
-
 60 min, about 30% loss of activity Panulirus interruptus
45
-
 60 min, about 40% loss of activity Panulirus interruptus
55
-
 20 min, about 80% loss of activity. 40 min, complete loss of activity Panulirus interruptus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.07
-
 succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Panulirus interruptus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Panulirus interruptus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 80% of maximal activity, pH 9.0: about 55% of maximal activity Panulirus interruptus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 Panulirus interruptus
3
-
 the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 Panulirus interruptus
5
-
 the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 Panulirus interruptus
8
-
 the enzyme conserves 100 % of its activity after 1 h Panulirus interruptus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8
-
 succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Panulirus interruptus