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Literature summary for 3.4.21.1 extracted from

  • Marquez-Rios, E.; Cota-Arriola, O.; Villalba-Villalba, A.G.; Ezquerra-Brauer, J.M.; Ocano-Higuera, V.M.; Lopez-Corona, B.E.; Torres-Arreola, W.
    Chymotrypsin isolation from jumbo squid (Dosidicus gigas) hepatopancreas Partial characterization and effect on muscle collagen (2016), Food Sci. Biotechnol., 25, 1011-1016 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.1

Application

Application Comment Organism
industry the enzyme possesses potential applications in the food, leather and silk industries where acidic or slightly alkaline conditions are needed Dosidicus gigas

Inhibitors

Inhibitors Comment Organism Structure
N-tosyl-L-phenylalanyl chloromethyl ketone
-
 Dosidicus gigas
phenyl methyl sulfonyl fluoride
-
 Dosidicus gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 SDS-PAGE Dosidicus gigas

Organism

Organism UniProt Comment Textmining
Dosidicus gigas
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dosidicus gigas

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Dosidicus gigas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.59
-
 substrate: succinyl-Ala-Ala-Pro-Phe-4-nitroanilide, pH 7.5, 25°C Dosidicus gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
muscle collagen + H2O moderate in vitro activity using muscle collagen as a substrate Dosidicus gigas ?
-
 ?
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Dosidicus gigas succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Dosidicus gigas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Dosidicus gigas

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 70 45°C: about 35% of maximal activity, 70°C: about 75% of maximal activity Dosidicus gigas

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 60 min, about 60% loss of activity Dosidicus gigas
45
-
 60 min, about 50% loss of activity Dosidicus gigas
55
-
 60 min, about 5% loss of activity Dosidicus gigas
65
-
 60 min, no loss of activity Dosidicus gigas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Dosidicus gigas

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity Dosidicus gigas

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 60 min, less than 20% loss of activity Dosidicus gigas