Any feedback?
Literature summary for 3.4.21.1 extracted from
- Homomultimeric protease in the hyperthermophilic bacterium Thermotoga maritima has structural and amino acid sequence homology to bacteriocins in mesophilic bacteria (1998), FEBS Lett., 440, 393-398 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, 50% loss of activity | Thermotoga maritima |  |
| SDS | 1%, activity is reduced 6fold | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | proteolytic activity is 1.5fold enhanced in the presence of 10 mM Ca2+ | Thermotoga maritima | |
| Co2+ | proteolytic activity is 1.4fold enhanced in the presence of 10 mM Co2+ | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 669000 | - |
gel filtration | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9WZP2 | - |
- |
| Thermotoga maritima DSM 3109 | Q9WZP2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the protease exhibits activity toward chymotrypsin and trypsin substrates. Substrate specificity to a variety of 7-amino-4-methylcoumarin-linked peptides in the order of decreasing activity: AAF, AFK, IIW, LY, GGF, AAPV. Little or no activity is detected on FVR, LLVY or GGR. No activity is noted on casein | Thermotoga maritima | ? | - |
? | |
| additional information | the protease exhibits activity toward chymotrypsin and trypsin substrates. Substrate specificity to a variety of 7-amino-4-methylcoumarin-linked peptides in the order of decreasing activity: AAF, AFK, IIW, LY, GGF, AAPV. Little or no activity is detected on FVR, LLVY or GGR. No activity is noted on casein | Thermotoga maritima DSM 3109 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homomultimer | x * 31000, the protease consists of a large globular assembly, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
half-life: 36 min | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Thermotoga maritima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
