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Literature summary for 3.4.21.1 extracted from

  • Zelazko, M.; Chrzanowska, J.; Polanowski, A.
    Pancreatic proteolytic enzymes of ostrich purified on immobilized protein inhibitors. Characterization of a new form of chymotrypsin (Chtr1) (2008), Comp. Biochem. Physiol. B, 151, 102-109.
    View publication on PubMed
Search for more literature for 3.4.21.1

Inhibitors

Inhibitors Comment Organism Structure
BPTI basic pancreatic trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected Struthio camelus
CMTI I Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect Struthio camelus
CMTI IV Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect Struthio camelus
CPGT I Cucurbita pepo var. giramontia inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed Struthio camelus
CPTI IV Cucurbita pepo inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed Struthio camelus
CyPTI VII trypsin inhibitors from Cyclanthera pedata, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed Struthio camelus
LUTI Linum usitatissimum inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect Struthio camelus
additional information affinity of Chtr1 to inhibitors containing Arg at P1 position. N-alpha-tosyl-L-lysine chloromethyl ketone (TLCK), Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition Struthio camelus
STI soybean trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected Struthio camelus
tosyl-L-phenylalanine chloromethyl ketone TPCK. Inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected Struthio camelus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide chymotrypsin 4 Struthio camelus
0.939
-
 N-alpha-benzoyl-DL-arginine-p-nitroanilide
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE Struthio camelus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Struthio camelus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Struthio camelus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-
pancreas
-
 Struthio camelus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
128
-
 activated extract Struthio camelus
500
-
 affinity chromatography on CMTI in the presence of 5 M NaCl, peak B Struthio camelus
1573
-
 affinity chromatography on CMTI (isolation of Chtr1) Struthio camelus
1800
-
 affinity chromatography on CMTI in the presence of 5 M NaCl, peak A Struthio camelus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specificity of Chtr1 reactive site is determined by hydrolysis of the oxidized bovine insulin B-chain Bos taurus ?
-
 ?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O BApNA Bos taurus ? + p-nitroaniline
-
 ?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O BApNA Struthio camelus ? + p-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Ala p-nitroanilide + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Ala + p-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
-
 Struthio camelus N-succinyl-Ala-Ala-Ala + p-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O SucAAPFpNA Bos taurus N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O SucAAPFpNA Struthio camelus N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
Chtr1
-
 Struthio camelus
Chtr2
-
 Struthio camelus
Chtr3
-
 Struthio camelus
Chtr4
-
 Struthio camelus
chymotrypsin
-
 Bos taurus
chymotrypsin
-
 Struthio camelus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.611
-
 N-alpha-benzoyl-DL-arginine-p-nitroanilide
-
 Bos taurus
10.58
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide chymotrypsin 4 Struthio camelus