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Literature summary for 3.4.21.1 extracted from

  • Spreti, N.; Mancini, M.V.; Germani, R.; Di Profio, P.; Savelli, G.
    Substrate effect on alpha-chymotrypsin activity in aqueous solutions of "big-head" ammonium salts (2008), J. Mol. Catal. B, 50, 1-6.
No PubMed abstract available
Search for more literature for 3.4.21.1

Activating Compound

Activating Compound Comment Organism Structure
cetyltributylammonium bromide promotes activity Bos taurus
tetrabutylammonium bromide promotes activity Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 N-succinyl-L-Ala-Ala-Phe-p-nitroanilide at 25°C Bos taurus
0.14
-
 N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide at 25°C Bos taurus
1.09
-
 N-succinyl-L-Phe-p-nitroanilide at 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-L-Ala-Ala-Phe + p-nitroaniline
-
 ?
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-L-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?
N-succinyl-L-Phe-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-L-Phe + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
alpha-chymotrypsin
-
 Bos taurus
alpha-CT
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 N-succinyl-L-Phe-p-nitroanilide at 25°C Bos taurus
7.8
-
 N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide at 25°C Bos taurus
39
-
 N-succinyl-L-Ala-Ala-Phe-p-nitroanilide at 25°C Bos taurus