Literature summary for 3.4.21.1 extracted from

Miao, Q.; Sun, Y.; Wei, T.; Zhao, X.; Zhao, K.; Yan, L.; Zhang, X.; Shu, H.; Yang, F.
Chymotrypsin B cached in rat liver lysosomes and involved in apoptotic regulation through a mitochondrial pathway (2008), J. Biol. Chem., 283, 8218-8228.

Search for more literature for 3.4.21.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
3,4-dichloroisocoumarin	complete inhibition at 0.2 mM	Rattus norvegicus
Aprotinin	partial inhibition at 0.1 mg/ml	Rattus norvegicus
chymostatin	complete inhibition at 0.25 mM	Rattus norvegicus
additional information	not inhibited by benzamide and L-1-chloro-3-(4-tosylamido)-7-amino-2-heptanone	Rattus norvegicus
N-p-tosyl-L-phenylalanine-chloromethyl ketone	complete inhibition at 5 mM	Rattus norvegicus
Phenylmethylsulfonylfluoride	complete inhibition at 2 mM	Rattus norvegicus
SERPINB3	partial inhibition	Rattus norvegicus
SERPINB4	partial inhibition	Rattus norvegicus
Soybean trypsin inhibitor	complete inhibition at 0.1 mg/ml	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Rattus norvegicus	5764	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	SDS-PAGE	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P07338	-	-

Purification (Commentary)

Purification (Comment)	Organism
SB-TI-Sepharose column chromatography	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
hepatoma cell	-	Rattus norvegicus	-
liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
BH3 interacting domain death agonist + H2O	-	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
chymotrypsin B	-	Rattus norvegicus
Ctrb	-	Rattus norvegicus
LBCP	-	Rattus norvegicus
lysosomal Bid cleavage protease	-	Rattus norvegicus

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Release 2023.1 (January 2023)