Activating Compound | Comment | Organism | Structure |
---|---|---|---|
p-octyloxybenzyltributylammonium bromide | maximal activation at 5 mM, inhibition at extended incubation | Bos taurus | |
tetrabutylammonium bromide | 400 mM, approx. 4fold activation | Bos taurus | |
tetrapentylammonium bromide | - |
Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-octyloxybenzyltributylammonium bromide | 5 mM, approx. 90% inactivation after 24 h | Bos taurus | |
p-octyloxybenzyltrimethylammonium bromide | 10 mM, complete inactivation | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.75 | Bos taurus | |
0.36 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.75, in the presence of 10 mM tetrabutylammonium bromide | Bos taurus | |
0.94 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.75, in the presence of 2.5 mM NaCl | Bos taurus | |
1 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.75, in the presence of 2 mM NaClO4 | Bos taurus | |
3.2 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.75, in the presence of 1000 mM tetrabutylammonium bromide | Bos taurus | |
6.1 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.75, in the presence of 400 mM tetrabutylammonium bromide | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Storage Stability | Organism |
---|---|
25°C, tetrabutylammonium bromide, 2 months, 30% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-glutaryl-L-Phe-p-nitroanilide + H2O | - |
Bos taurus | N-glutaryl-L-Phe + p-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.8 | Bos taurus | |
0.017 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.8, in the presence of 10 mM tetrabutylammonium bromide | Bos taurus | |
0.025 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.8, in the presence of 1000 mM tetrabutylammonium bromide | Bos taurus | |
0.034 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.8, in the presence of 2 mM NaClO4 | Bos taurus | |
0.039 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.8, in the presence of 2.5 mM NaCl | Bos taurus | |
0.31 | - |
N-glutaryl-L-Phe-p-nitroanilide | 25°C, pH 7.8, in the presence of 400 mM tetrabutylammonium bromide | Bos taurus |