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Literature summary for 3.4.19.9 extracted from

  • Ramos-Parra, P.; Urrea-Lopez, R.; Diaz de la Garza, R.
    Folate analysis in complex food matrices: Use of a recombinant Arabidopsis gamma-glutamyl hydrolase for folate deglutamylation (2013), Food Res. Int., 54, 177-185.
No PubMed abstract available
Search for more literature for 3.4.19.9

Application

Application Comment Organism
analysis folate analysis. Isoform GGH2 is able to completely deconjugate folates using 50-100 microg/g of sample for 1 h at 37°C in all matrices tested. It removes the tails of folylpolyglutamates from tomato fruit, black bean and peanut seeds, and alfalfa sprouts extracts Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O65355 isoform GGH2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyltetrahydrofolate glutamate + H2O
-
 Arabidopsis thaliana 5,10-methenyltetrahydrofolate + L-glutamate
-
 ?
5,10-methenyltetrahydrofolate heptaglutamate + 7 H2O
-
 Arabidopsis thaliana 5,10-methenyltetrahydrofolate + 7 L-glutamate
-
 ?
5,10-methenyltetrahydrofolate hexaglutamate + 6 H2O
-
 Arabidopsis thaliana 5,10-methenyltetrahydrofolate + 6 L-glutamate
-
 ?
5-formyltetrahydrofolate glutamate + H2O
-
 Arabidopsis thaliana 5-formyltetrahydrofolate + L-glutamate
-
 ?
5-formyltetrahydrofolate heptaglutamate + 7 H2O
-
 Arabidopsis thaliana 5-formyltetrahydrofolate + 7 L-glutamate
-
 ?
5-formyltetrahydrofolate hexaglutamate + 6 H2O
-
 Arabidopsis thaliana 5-formyltetrahydrofolate + 6 L-glutamate
-
 ?
5-methyltetrahydrofolate glutamate + H2O
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + L-glutamate
-
 ?
5-methyltetrahydrofolate heptaglutamate + 7 H2O
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + 7 L-glutamate
-
 ?
5-methyltetrahydrofolate hexaglutamate + 6 H2O
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + 6 L-glutamate
-
 ?
additional information isoform GGH2 alone is able to completely deconjugate folates using 50-100 microg/g of sample for 1 h at 37°C in all matrices tested. It removes the tails of folylpolyglutamates from tomato fruit, black bean and peanut seeds, and alfalfa sprouts extracts Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
At1g78680
-
 Arabidopsis thaliana
GGH2
-
 Arabidopsis thaliana